Testing Data
phospho-g-Catenin (Tyr-550) Peptide
phospho-gamma-Catenin (Tyr-550) Blocking Peptide
Synonyms
phospho-g-Catenin (Tyr-550) Peptide; phospho-gamma-Catenin (Tyr-550) Blocking Peptide; phospho-g-Catenin (Tyr-550) Peptide peptide
Specificity
The peptide is specifically recognized by gamma-Catenin (Tyr-550) phospho-specific antibody (CP1121) in ELISA, and has been shown to block the reactivity of CP1121 during Western blot. In addition, the peptide is recommended for use in blocking CP1121 reactivity in immunocytochemistry.
Form/Format
Blocking Peptide is supplied in 50ul phosphate-buffered saline and 0.05% sodium azide.
Sequence
Peptide Sequence: Phospho-gamma-Catenin (Tyr-550) synthetic peptide corresponds to amino acid residues around tyrosine 550 of human gamma-Catenin. This peptide sequence is highly conserved in rat and mouse gamma-Catenin.
Application Notes
Antibody Blocking: 1 ug/ml
ELISA: 10-100 ng/well
ELISA: 10-100 ng/well
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Testing Data
Testing Data
Related Product Information for phospho-g-Catenin (Tyr-550) Peptide peptide
Plakoglobin (gamma-catenin) is a catenin family member that was identified as a component of desmosomes. It associates with various cell adhesion related proteins, such as desmoglein and cadherins. gamma-Catenin has high homology to beta-catenin and, like beta-catenin, it can associate with the cadherins, E-cadherin and N-cadherin. It has been proposed that one molecule of alpha catenin and at least one molecule of beta-catenin and gamma-catenin simultaneously bind to a single cadherin molecule. A 19-amino acid sequence of desmoglein was found to be critical for binding of gamma-catenin. This region has significant homology to the catenin-binding domain of classical cadherins, thus suggesting a common mechanism for gamma-catenin localization at both adherens junctions and desmosomes. Phosphorylation of tyrosine residues in gamma-catenin can modify its interactions with other proteins. Phosphorylation of tyrosine 644 decreases gamma- catenin association with alpha-catenin, but increases binding to desmoplakin. Fer kinase can phosphorylate tyrosine 550, which increases gamma-catenin binding to alpha-catenin. Thus, tyrosinephosphorylation may be important for regulation of gamma-catenin protein-protein interactions within desmosomal complexes.
References
• Ozawa, M. et al. (1990) Proc. Natl. Acad. Sci. USA 87:4246.
• Roura, S. et al. (1999) J Biol Chem. 274(51) :36734.
• Piedra, J. et al. (2003) Mol. Cell. Biol. 23(7):2287-2297.
• Brembeck, F.H. et al. (2004) Genes Dev. 18(18):2225-2230.
• Roura, S. et al. (1999) J Biol Chem. 274(51) :36734.
• Piedra, J. et al. (2003) Mol. Cell. Biol. 23(7):2287-2297.
• Brembeck, F.H. et al. (2004) Genes Dev. 18(18):2225-2230.
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Product Notes
The phospho-g-Catenin (Tyr-550) Peptide (Catalog #AAA474037) is a Peptide and is intended for research purposes only. The product is available for immediate purchase. Antibody Blocking: 1 ug/ml ELISA: 10-100 ng/well. Researchers should empirically determine the suitability of the phospho-g-Catenin (Tyr-550) Peptide for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Peptide Sequence: Phospho-ga mma-Cateni n (Tyr-550) synthetic peptide correspond s to amino acid residues around tyrosine 550 of human gamma-Cate nin. This peptide sequence is highly conserved in rat and mouse gamma-Cate nin. It is sometimes possible for the material contained within the vial of "phospho-g-Catenin (Tyr-550) Peptide, Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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