phospho-PDK1 (Tyr-9) Peptide | PDK1 peptide

phospho-PDK1 (Tyr-9) Peptide

Cat. Num. AAA474051

• Gene Names: PDK1; PDK1
• Synonyms: phospho-PDK1 (Tyr-9) Peptide; PDK1 peptide

• Specificity: The peptide is specifically recognized by anti-PDK1 (Tyr-9) phospho-specific antibody (PP1431) in ELISA, and has been shown to block the reactivity of PP1431 during Western blot. In addition, the peptide is recommended for use in blocking PP1431 reactivity in immunocytochemistry.
• Form/Format: Blocking Peptide is supplied in 50ul phosphate-buffered saline and 0.05% sodium azide.
• Sequence: Peptide Sequence: Phospho-PDK1 (Tyr-9) synthetic peptide corresponds to amino acids surrounding tyrosine 9 of human PDK1. This sequence is highly conserved in rat and mouse PDK1.
• Sequence Length: 436

• Application Notes: Blocking: 1:1,000; ELISA: 50 ng/well
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Testing Data

Related Product Information for PDK1 peptide

3-Phosphoinositide-dependent kinase 1 (PDK1), also known as PKB kinase, was identified as the activator of the survival kinase Akt/PKB. Several important substrates of PDK1 include p70S6 kinase, PKAs, PKCs, SGKs, RSKs, and PAKs. PDK1 is a member of the AGC superfamily of serine/threonine kinases. Through the phosphorylation of downstream kinases, like Akt, PDK1 has been shown to be involved in several different cell functions, such as protein synthesis, cell survival, glucose metabolism, and cell adhesion and migration. The regulation of PDK1 occurs through lipid second messengers and phosphorylation. Multiple serine sites are phosphorylated on PDK1. Serine 241 phosphorylation is required for PDK1 activity, while serine 396 has been implicated in PDK1 nuclear translocation. Tyrosine phosphorylation may also regulate PDK1 activity. Tyrosines 9 and 373/376 are phosphorylated by c-Src in vitro. Tyr-373/Tyr-376 are important for PDK1 activity, while Tyr-9 phosphorylation permits Tyr-373/Tyr-376 phosphorylation by c-Src. In addition, Tyr-9 may be important during angiotensin-II-induced focal adhesion formation.

References

• Park, J. et al. (2001) J Biol. Chem. 276(40):37459.
• Taniyama, Y. et al. (2003) Mol Cell. Biol. 23(22):8019.
• Scheid, M.P. et al. (2005) Mol. Cell. Biol. 25(6):2347.

NCBI and Uniprot Product Information

• NCBI GI #: 24660128
• NCBI GeneID: 5163
• NCBI Accession #: AAH39158.1
• NCBI GenBank Nucleotide #: BC039158.1
• UniProt Accession #: Q15118; B2R6T1; D3DPD8; Q308M4; Q53R49
• Molecular Weight: 49,244 Da
• NCBI Official Full Name: PDK1 protein
• NCBI Official Synonym Full Names: pyruvate dehydrogenase kinase, isozyme 1
• NCBI Official Symbol: PDK1
• NCBI Official Synonym Symbols: PDK1
• NCBI Protein Information: pyruvate dehydrogenase kinase, isozyme 1; OTTHUMP00000163127; OTTHUMP00000205076; OTTHUMP00000205077; OTTHUMP00000205082; pyruvate dehydrogenase kinase, isoenzyme 1; mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial
• UniProt Protein Name: [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial
• Protein Family: [Pyruvate dehydrogenase
• UniProt Gene Name: PDK1
• UniProt Entry Name: PDK1_HUMAN

NCBI Description

Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. [provided by RefSeq]

Uniprot Description

Function: Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism. Ref.6

Catalytic activity: ATP + [pyruvate dehydrogenase (acetyl-transferring)] = ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate.

Enzyme regulation: Inhibited by AZD7545, dichloroacetate and radicicol. Ref.6

Subcellular location: Mitochondrion matrix.

Tissue specificity: Expressed predominantly in the heart.

Sequence similarities: Belongs to the PDK/BCKDK protein kinase family.Contains 1 histidine kinase domain.