Rabbit b-Catenin (pY280) Antibody | anti-b-Catenin (pY280) antibody

Rabbit anti Phosph-beta-Catenin (Tyr280) antibody

Cat. Num. AAA462211

• Gene Names: ctnnb1; ctnnb; id:ibd2058; wu:fb73e10; wu:fi81c06; wu:fk25h01
• Reactivity: Human, Rat, Mouse, Chicken, Canine
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
• Synonyms: b-Catenin (pY280); Rabbit anti Phosph-beta-Catenin (Tyr280) antibody; Rabbit anti-beta-Catenin (pY280); anti-b-Catenin (pY280) antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse, Chicken, Canine
• Specificity: This antibody recognizes ~88 kDa of human beta-Catenin protein. It also reacts with mouse and rat. The other species are not tested.
• Purity/Purification: The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
• Form/Format: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
• Concentration: Size: 100 ug/200 ul (varies by lot)

• Applicable Applications for anti-b-Catenin (pY280) antibody: ELISA, Western Blot, Immunohistochemistry
• Application Notes: Western Blot: 0.1-1 ug/ml; ELISA: 0.01-0.1 ug/ml; Immunoprecipitation: 2-5 ug/ml; Immunohistochemistry: 2-5 ug/ml
• Antigen Preparation: A synthetic peptide corresponding to the epitope EPYGL at the phosphorylation site Tyr280 of human beta-catenin.
• Positive Control: HELA cell lysate
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Testing Data

Related Product Information for anti-b-Catenin (pY280) antibody

Beta-catenin is a cytosolic, 88 kDa, 781 amino acid protein belongs to the beta-catenin family. The N-terminus domain, containing the binding site and the phosphorylation sites. Beta-Catenin serves as a link between cytoskeleton actin and transmembrane cadherin (s). It is believed to contribute to tight cell-to-cell adhesion. It can enter the nucleus and interact with the TCF/LEF family of transcription factors, initiating gene expression. Normally, beta-catenin transcriptional activity is suppressed by a Ser/Thr kinase termed GSK3beta and/or Casein Kinase I (CK1). Kinases are constitutively active and phosphorylates beta-catenin at multiple sites, including S33 and S37, Y96, Y228, Y280 etc. Phosphorylation of beta-catenin targets the molecule for degradation via a ubiquination-mediated pathway. GSK3beta activity can be blocked by upstream signaling events such as Wnt-Frizzled interaction. This inhibits GSK3beta, allowing unphosphorylated beta-catenin to enter the nucleus and initiate gene activation. The phosphorylation of beta-catenin might contribute to tumorigenesis.

References

Park, C.S. et al. (2004) J. Biol. Chem. 279:19592.

NCBI and Uniprot Product Information

• NCBI GI #: 1125100
• NCBI GeneID: 30265
• UniProt Accession #: Q7ZU14; Q90424
• Molecular Weight: 88 kDa
• NCBI Official Full Name: b-catenin
• NCBI Official Synonym Full Names: catenin (cadherin-associated protein), beta 1
• NCBI Official Symbol: ctnnb1
• NCBI Official Synonym Symbols: ctnnb; id:ibd2058; wu:fb73e10; wu:fi81c06; wu:fk25h01
• NCBI Protein Information: catenin beta-1; beta-catenin
• UniProt Protein Name: Catenin (Cadherin-associated protein), beta 1
• UniProt Gene Name: ctnnb1
• UniProt Entry Name: Q7ZU14_DANRE