Testing Data
Rabbit b-Catenin (Tyr-142) Polyclonal Antibody | anti-ctnnb1 antibody
Anti-beta-Catenin (Tyr-142), phospho-specific [gamma-Catenin (Tyr-133)]
Gene Names
ctnnb1; ctnnb; id:ibd2058; wu:fb73e10; wu:fi81c06; wu:fk25h01
Reactivity
Human, Rat, Mouse
Applications
ELISA, Western Blot
Purity
Affinity Purified
Synonyms
b-Catenin (Tyr-142); Polyclonal Antibody; Anti-beta-Catenin (Tyr-142); phospho-specific [gamma-Catenin (Tyr-133)]; phospho-specific; Catenin beta; anti-ctnnb1 antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Specificity
This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose before affinity purification using phospho- beta -Catenin (Tyr-142) peptide (without carrier). The antibody detects a 92kDa* protein corresponding to the molecular mass of beta -Catenin on SDS-PAGE immunoblots of Hct116 src transformed cells treated with pervanadate, but not in control cells. Similar results were observed in other pervanadate-treated cell lines, such as A431 and human endothelial cells.
Purity/Purification
Affinity Purified
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-ctnnb1 antibody
ELISA, Western Blot
Application Notes
ELISA: 1:2000
Western Blot: 1:500
ICC: 1:100
Western Blot: 1:500
ICC: 1:100
Immunogen
Phospho-beta-Catenin (Tyr-142) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 142 of human beta-Catenin. This peptide sequence has one amino acid difference from a sequence around tyrosine 133 of human gamma-Catenin. These human sequences are highly conserved in rat and mouse beta-Catenins.
Preparation and Storage
Store at -20 degree C. Do not aliquot. Stable for 1 year.
Testing Data
Testing Data
Immunocytochemistry
Immunocytochemistry
Related Product Information for anti-ctnnb1 antibody
beta-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of ECadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of beta-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between alpha and beta-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of beta-Catenin. Src family kinases can also phosphorylate tyrosine 654 in the C terminal armadillo repeat of beta-Catenin. This phosphorylation regulates beta-Catenin binding to E cadherin. Thus, sitespecific tyrosine phosphorylation of beta-Catenin may regulate specific protein-protein interactions leading to changes in cell adhesion.
References
• Brembeck, F.H. et al. (2004) Genes Dev. 18(18):2225-2230.
• Ozawa, M. et al. (1990) Proc. Natl. Acad. Sci. USA 87:4246.
• Piedra, J. et al. (2003) Mol. Cell. Biol. 23(7):2287-2297.
• Ozawa, M. et al. (1990) Proc. Natl. Acad. Sci. USA 87:4246.
• Piedra, J. et al. (2003) Mol. Cell. Biol. 23(7):2287-2297.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
85,520 Da
NCBI Official Full Name
b-catenin
NCBI Official Synonym Full Names
catenin (cadherin-associated protein), beta 1
NCBI Official Symbol
ctnnb1
NCBI Official Synonym Symbols
ctnnb; id:ibd2058; wu:fb73e10; wu:fi81c06; wu:fk25h01
NCBI Protein Information
catenin beta-1; beta-catenin
UniProt Protein Name
Catenin (Cadherin-associated protein), beta 1
Protein Family
UniProt Gene Name
ctnnb1
UniProt Entry Name
Q7ZU14_DANRE
Research Articles on ctnnb1
Similar Products
Product Notes
The ctnnb1 ctnnb1 (Catalog #AAA472004) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-beta-Catenin (Tyr-142), phospho-specific [gamma-Catenin (Tyr-133)] reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's b-Catenin (Tyr-142) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. ELISA: 1:2000 Western Blot: 1:500 ICC: 1:100. Researchers should empirically determine the suitability of the ctnnb1 ctnnb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "b-Catenin (Tyr-142), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
