Testing Data
Rabbit B-Catenin Polyclonal Antibody | anti-ctnnb1 antibody
B-Catenin Antibody
Gene Names
ctnnb1; ctnnb; id:ibd2058; wu:fb73e10; wu:fi81c06; wu:fk25h01
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry
Purity
Affinity Chromatography
Synonyms
B-Catenin; Polyclonal Antibody; B-Catenin Antibody; Affinity Purified Rabbit Polyclonal B-Catenin Antibody; Polyclonal B-Catenin antibody; Anti-B-Catenin antibody; Catenin beta antibody; Beta Catenin antibody; Catenin antibody; anti-ctnnb1 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Purified polyclonal CTNNB1 antibody specific for Human CTNNB1 for use in Immunohistochemistry and Western blot.
Purity/Purification
Affinity Chromatography
Form/Format
Lyophilized Form
Sequence Length
780
Applicable Applications for anti-ctnnb1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Frozen
Immunogen
B-Catenin antibody was raised in Rabbit using a synthetic peptide corresponding to a sequence at the C-terminal of human Catenin beta, identical to the related rat and Rabbit sequence as the immunogen.
Post-Translational Modifications
Phosphorylation at Ser-552 by AMPK promotes stabilizion of the protein, enhancing TCF/LEF-mediated transcription. Phosphorylated by NEK2. EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding. Phosphorylated on Ser-33 and Ser-37 by HIPK2. This phosphorylation triggers proteasomal degradation. Phosphorylation on Ser-191 and Ser-246 by CDK5. Phosphorylation by CDK2 regulates insulin internalization. Phosphorylation by PTK6 at Tyr-64, Tyr-142, Tyr-331 and/or Tyr-333 with the predominant site at Tyr-64 is not essential for inhibition of transcriptional activity. Ubiquitination occurs at Lys19, Lys133, Lys158, Lys170, Lys233, Lys335, Lys345, Lys354, Lys435 and Lys508.
Expression
CTNNB1 is heavily expressed during transcription events. The majority of CTNNB1 is localized to the cell membrane. In interphase, colocalizes with CROCC between CEP250 puncta at the proximal end of centrioles, and this localization is dependent on CROCC and CEP250. In mitosis, when NEK2 activity increases, it localizes to centrosomes at spindle poles independent of CROCC. Colocalizes with CDK5 in the cell-cell contacts and plasma membrane of undifferentiated and differentiated neuroblastoma cells. Colocalized with RAPGEF2 and TJP1 at cell-cell contacts.
Preparation and Storage
Store at 4 degree C for short term storage. For long term storage aliquot and store at -20 degree C. Avoid repeated freeze/thaw cycles.
Testing Data
Testing Data
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded section of Human Mammary Cancer Tissue)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded section of Human Mammary Cancer Tissue)
Western Blot (WB)
(Lane 1: MM453 Cell Lysate; Lane 2: MCF-7 Cell Lysate; Lane 3: Hela Cell Lysate)
Western Blot (WB)
(Lane 1: MM453 Cell Lysate; Lane 2: MCF-7 Cell Lysate; Lane 3: Hela Cell Lysate)
Related Product Information for anti-ctnnb1 antibody
Description: CTNNB1 is composed of 781 amino acids with a molecular weight of 85497 Da. There are 12 ARM repeats between positions 151 and 666.
Biological Significance: CTNNB1 is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. CTNNB1 is a key downstream component of the canonical Wnt signaling pathway.
Implications In Disease: Defects in CTNNB1 are associated with colorectal cancer. Genetic alterations are often associated with progression from premalignant lesion (adenoma) to invasive adenocarcinoma. Risk factors for cancer of the colon and rectum include colon polyps, long-standing ulcerative colitis, and genetic family history. Mutations in this gene are also a cause of pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer.
Biological Significance: CTNNB1 is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. CTNNB1 is a key downstream component of the canonical Wnt signaling pathway.
Implications In Disease: Defects in CTNNB1 are associated with colorectal cancer. Genetic alterations are often associated with progression from premalignant lesion (adenoma) to invasive adenocarcinoma. Risk factors for cancer of the colon and rectum include colon polyps, long-standing ulcerative colitis, and genetic family history. Mutations in this gene are also a cause of pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer.
Product Categories/Family for anti-ctnnb1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
b-catenin
NCBI Official Synonym Full Names
catenin (cadherin-associated protein), beta 1
NCBI Official Symbol
ctnnb1
NCBI Official Synonym Symbols
ctnnb; id:ibd2058; wu:fb73e10; wu:fi81c06; wu:fk25h01
NCBI Protein Information
catenin beta-1
Protein Family
Research Articles on ctnnb1
Similar Products
Product Notes
The ctnnb1 (Catalog #AAA5312614) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's B-Catenin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Frozen. Researchers should empirically determine the suitability of the ctnnb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B-Catenin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
