Western Blot (WB)
(The cell lysate derived HepG2 was in 12% SDS-PAGE, transferred onto NC membrane, and immunoblotted by Rabbit anti PLAC1 (MBS460437) at 1:500. An immunoreactive major band was observed at ~22 kDa.)
Rabbit PLAC1 Polyclonal Antibody | anti-PLAC1 antibody
Rabbit anti PLAC1 Polyclonal Antibody
Gene Names
PLAC1; CT92; OOSP2L
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The Rabbit IgG is purified by Epitope Affinity Purification.
Synonyms
PLAC1; Polyclonal Antibody; Rabbit anti PLAC1 Polyclonal Antibody; placenta-specific protein 1; anti-PLAC1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
This antibody recognizes ~22 kDa of PLAC1 protein. It cross-reacts to human, rat and mouse. The other species are not tested.
Purity/Purification
The Rabbit IgG is purified by Epitope Affinity Purification.
Form/Format
Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
Applicable Applications for anti-PLAC1 antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunohistochemistry: 0.5-2 ug/ml
Optimal dilutions should be determined by researchers for the specific applications.
ELISA: 0.01-0.1 ug/ml
Immunohistochemistry: 0.5-2 ug/ml
Optimal dilutions should be determined by researchers for the specific applications.
Preparation
A synthetic peptide corresponding to the C-terminus of mouse PLAC1 protein (from 145aa-175aa). This sequence is identical to human, rat and mouse.
Positive Control
HepG2
Cellular location
Membrane
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C to 8 degree C for three months without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Western Blot (WB)
(The cell lysate derived HepG2 was in 12% SDS-PAGE, transferred onto NC membrane, and immunoblotted by Rabbit anti PLAC1 (MBS460437) at 1:500. An immunoreactive major band was observed at ~22 kDa.)
Western Blot (WB)
(The cell lysate derived HepG2 was in 12% SDS-PAGE, transferred onto NC membrane, and immunoblotted by Rabbit anti PLAC1 (MBS460437) at 1:500. An immunoreactive major band was observed at ~22 kDa.)
Related Product Information for anti-PLAC1 antibody
The PLAC1, a placenta-specific gene, which encodes a putative cell surface protein, is highly expressed in placenta, testis, cancer cell lines and lung tumors. PLAC1 is exclusively expressed by cells of trophoblastic lineage in the mouse, and maps to a region of the X-chromosome known to be important in placenta growth. PLAC1 is also expressed in human hepatocellular cancer tissues as well as in several other types of cancer tissues and/or tumor cell lines. PLAC1 represents a new class of tumor associated antigen with restricted expression in placenta and cancer tissues, that may serve as a target for cancer vaccination.
References
Dong XY, et al. Plac1 is a tumor-specific antigen capable of eliciting spontaneous antibody responses in human cancer patients. Int J Cancer. 122 (9):2038-43 (2008).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~22 kDa
NCBI Official Full Name
placenta-specific protein 1
NCBI Official Synonym Full Names
placenta specific 1
NCBI Official Symbol
PLAC1
NCBI Official Synonym Symbols
CT92; OOSP2L
NCBI Protein Information
placenta-specific protein 1
UniProt Protein Name
Placenta-specific protein 1
Protein Family
UniProt Gene Name
PLAC1
Uniprot Description
PLAC1: May play a role in placental development. Belongs to the PLAC1 family.
Protein type: Cancer Testis Antigen (CTA); Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: Xq26.3
Biological Process: placenta development
Research Articles on PLAC1
Similar Products
Product Notes
The PLAC1 plac1 (Catalog #AAA460437) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti PLAC1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PLAC1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). Western Blot: 0.1-1 ug/ml ELISA: 0.01-0.1 ug/ml Immunohistochemistry: 0.5-2 ug/ml Optimal dilutions should be determined by researchers for the specific applications. Researchers should empirically determine the suitability of the PLAC1 plac1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLAC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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