Rabbit Legumain Polyclonal Antibody | anti-LGMN antibody

Rabbit anti Legumain Polyclonal Antibody

Cat. Num. AAA460341

• Gene Names: LGMN; AEP; LGMN1; PRSC1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Epitope Affinity Purification
• Synonyms: Legumain; Polyclonal Antibody; Rabbit anti Legumain Polyclonal Antibody; LGMN; AEP; PRSC1; anti-LGMN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody recognizes ~50 kDa of Legumain protein. It reacts with human, rat and mouse. The other species are not tested.
• Purity/Purification: Purified by Epitope Affinity Purification
• Form/Format: Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Sequence Length: 433

• Applicable Applications for anti-LGMN antibody: ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: ELISA: 0.01-0.1 ug/ml; Immunohistochemistry: 0.5-2 ug/ml; Immunoprecipitation: 2-5 ug/ml; Western Blot: 0.1-1 ug/ml ; ; Optimal dilutions should be determined by researchers for the specific applications.
• Antigen Preparation: A synthetic peptide corresponding to the internal sequence (a portion from 100aa-190aa) of human Legumain protein. This sequence is identical to mouse, human and rat.
• Positive Control: HT-29
• Cellular Location: Cytoplasmic
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C. The antibodies can be stored at 2°C-8°C for three months without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Western Blot (WB)

(Western Blot: The cell lysate derived from HT-29 (lane 1), Hela (Lane 2) and mouse kidney (lane 3) were resolved onto 10% SDS-PAGE, transferred onto NC membrane, and immunoblotted by Rb anti Legumain antibody (MBS460341) at 1:500. An immunoreactive band around ~50 kDa was observed.)

Immunohistochemistry (IHC)

(IHC: Human ovary carcinoma stained with Anti-Legumain (MBS460341) 1:200 dilution. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)

Related Product Information for anti-LGMN antibody

Legumain, a 433 amino acid protein with 17 aa signal sequence, is a cystein protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This novel asparaginyl endopeptidase has been observed to be highly expressed in several types of tumors including colorectal cancer.

References

Murthy, R.V. et al, Legumain Expression in Relation to Clinicopathologic and Biological Variables in Colorectal Cancer. Clinical Cancer Research. 11 (6), 2293-2299 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 56682964
• NCBI GeneID: 5641
• NCBI Accession #: NP_001008530.1
• NCBI GenBank Nucleotide #: NM_001008530.2
• UniProt Accession #: Q99538; O00123; Q86TV2; Q86TV3; Q9BTY1
• Molecular Weight: ~50 kDa
• NCBI Official Full Name: legumain preproprotein
• NCBI Official Synonym Full Names: legumain
• NCBI Official Symbol: LGMN
• NCBI Official Synonym Symbols: AEP; LGMN1; PRSC1
• NCBI Protein Information: legumain
• UniProt Protein Name: Legumain
• Protein Family: Legumain
• UniProt Gene Name: LGMN
• UniProt Synonym Gene Names: PRSC1

NCBI Description

This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. [provided by RefSeq, Jul 2008]

Uniprot Description

LGMN: Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. Belongs to the peptidase C13 family.

Protein type: EC 3.4.22.34; Protease

Chromosomal Location of Human Ortholog: 14q32.12

Cellular Component: lysosomal lumen; lysosome

Molecular Function: cysteine-type endopeptidase activity; peptidase activity

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; negative regulation of neuron apoptosis; proteolysis; proteolysis involved in cellular protein catabolic process; receptor catabolic process; renal system process; toll-like receptor signaling pathway; vacuolar protein processing; vitamin D metabolic process