Western Blot (WB)
(Western Blot: The whole cell lysate derived from HT-29 cell lysate was separated in 10% SDS-PAGE, transferred onto NC membrane, and immunoblotted by Rabbit anti –Tnc (Cat#MBS460350) antibody at 1:500 . An immunoreactive band around ~240 kDa was observed.)
Rabbit Tenascin C Polyclonal Antibody | anti-TNC antibody
Rabbit anti Tenascin C Polyclonal Antibody
Flow Cytometry: 10-20 ug/ml
Immunohistochemistry: 10-20 ug/ml
Immunoprecipitation: 2-5 ug/ml
Western Blot: 0.1-1 ug/ml
Optimal dilutions should be determined by researchers for the specific applications.
Western Blot (WB)
(Western Blot: The whole cell lysate derived from HT-29 cell lysate was separated in 10% SDS-PAGE, transferred onto NC membrane, and immunoblotted by Rabbit anti –Tnc (Cat#MBS460350) antibody at 1:500 . An immunoreactive band around ~240 kDa was observed.)
Western Blot (WB)
(Western Blot: The whole cell lysate derived from HT-29 cell lysate was separated in 10% SDS-PAGE, transferred onto NC membrane, and immunoblotted by Rabbit anti –Tnc (Cat#MBS460350) antibody at 1:500 . An immunoreactive band around ~240 kDa was observed.)
Immunohistochemistry (IHC)
(IHC: Human lung tissue stained with Anti-Tnc antibody, (Cat# MBS460350) at 1:100 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
Immunohistochemistry (IHC)
(IHC: Human lung tissue stained with Anti-Tnc antibody, (Cat# MBS460350) at 1:100 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an extracellular matrix protein with a spatially and temporally restricted tissue distribution. This protein is homohexameric with disulfide-linked subunits, and contains multiple EGF-like and fibronectin type-III domains. It is implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity, and neuronal regeneration. [provided by RefSeq, Jul 2011]
Uniprot Description
TNC: Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. Belongs to the tenascin family. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 9q33.1
Cellular Component: endoplasmic reticulum lumen; extracellular matrix; extracellular region; extracellular space; focal adhesion; membrane
Molecular Function: syndecan binding
Biological Process: cellular protein metabolic process; extracellular matrix organization and biogenesis; osteoblast differentiation; post-translational protein modification; response to wounding
Disease: Deafness, Autosomal Dominant 56
Research Articles on TNC
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Product Notes
The TNC tnc (Catalog #AAA460350) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Tenascin C Polyclonal Antibody reacts with Mouse, Rat, Human and may cross-react with other species as described in the data sheet. AAA Biotech's Tenascin C can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). ELISA: 0.01-0.1 ug/ml Flow Cytometry: 10-20 ug/ml Immunohistochemistry: 10-20 ug/ml Immunoprecipitation: 2-5 ug/ml Western Blot: 0.1-1 ug/ml Optimal dilutions should be determined by researchers for the specific applications. Researchers should empirically determine the suitability of the TNC tnc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tenascin C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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