Mouse Coagulation Factor IX (F9) ELISA Kit | F9 elisa kit

Mouse Coagulation Factor IX (F9) ELISA Kit

Cat. Num. AAA451808

• Gene Names: F9; Cf9; Cf-9; AW111646
• Reactivity: Mouse
• Synonyms: Coagulation Factor IX (F9); Mouse Coagulation Factor IX (F9) ELISA Kit; HEMB; FIX; GLA Domain; PTC; Anti Hemophilic Factor B; Christmas Factor; Plasma Thromboplastic Component; Christmas Disease; Hemophilia B; F9 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of F9. No significant cross-reactivity or interference between F9 and analogues was observed.
• Sequence Length: 433

• Samples: Plasma.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.067ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level F9 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level F9 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for F9 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of F9 in mouse plasma.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to F9. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to F9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain F9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of F9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 802084014
• NCBI GeneID: 14071
• NCBI Accession #: NP_001292726.1
• NCBI GenBank Nucleotide #: NM_001305797.1
• UniProt Accession #: P16294; Q3UES1
• Molecular Weight: 52,978 Da
• NCBI Official Full Name: coagulation factor IX isoform 2
• NCBI Official Synonym Full Names: coagulation factor IX
• NCBI Official Symbol: F9
• NCBI Official Synonym Symbols: Cf9; Cf-9; AW111646
• NCBI Protein Information: coagulation factor IX
• UniProt Protein Name: Coagulation factor IX
• Protein Family: Coagulation factor
• UniProt Gene Name: F9
• UniProt Synonym Gene Names: Cf9

NCBI Description

This gene encodes a vitamin K-dependent serine protease that plays a critical role in the intrinsic pathway of blood coagulation. The encoded protein is an inactive zymogen that is activated by coagulation factor XIa to generate factor IXa, a heterodimer containing heavy and light chains. In association with factor VIII, membrane phospholipids and calcium ions, factor IXa cleaves the inactive zymogen factor X to generate active factor Xa. Genetic deletion of this gene in mice results in a severe bleeding phenotype. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2015]

Uniprot Description

Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca2+ ions, phospholipids, and factor VIIIa.