Mouse Coagulation Factor IX (F9) ELISA Kit | F9 elisa kit

Mouse Coagulation Factor IX (F9) ELISA Kit

Cat. Num. AAA2024000

• Gene Names: F9; FIX; P19; PTC; HEMB; THPH8
• Reactivity: Mouse
• Synonyms: Coagulation Factor IX (F9); Mouse Coagulation Factor IX (F9) ELISA Kit; HEMB; FIX; GLA Domain; PTC; Anti Hemophilic Factor B; Christmas Factor; Plasma Thromboplastic Component; Christmas Disease; Hemophilia B; F9 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Coagulation Factor IX (F9).; No significant cross-reactivity or interference between Coagulation Factor IX (F9) and analogues was observed.
• Sequence Length: 423

• Assay Type: Double-antibody Sandwich
• Samples: Plasma
• Detection Range: 1.56-100ng/mL
• Sensitivity: < 0.67ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coagulation Factor IX (F9) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coagulation Factor IX (F9) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for F9 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor IX (F9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coagulation Factor IX (F9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coagulation Factor IX (F9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coagulation Factor IX (F9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for F9 elisa kit

Signal transduction; Hematology

NCBI and Uniprot Product Information

• NCBI GI #: 4503649
• NCBI GeneID: 2158
• NCBI Accession #: NP_000124.1
• NCBI GenBank Nucleotide #: NM_000133.3
• UniProt Accession #: P00740; Q5FBE1; Q5JYJ8; A8K9N4; F2RM36
• Molecular Weight: 47,618 Da
• NCBI Official Full Name: coagulation factor IX preproprotein
• NCBI Official Synonym Full Names: coagulation factor IX
• NCBI Official Symbol: F9
• NCBI Official Synonym Symbols: FIX; P19; PTC; HEMB; THPH8
• NCBI Protein Information: coagulation factor IX
• UniProt Protein Name: Coagulation factor IX
• Protein Family: Coagulation factor
• UniProt Gene Name: F9
• UniProt Synonym Gene Names: PTC
• UniProt Entry Name: FA9_HUMAN

NCBI Description

This gene encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease. [provided by RefSeq, Jul 2008]

Uniprot Description

F9: Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa. Defects in F9 are the cause of recessive X-linked hemophilia B (HEMB); also known as Christmas disease. Mutations in position 43 (Oxford-3, San Dimas) and 46 (Cambridge) prevents cleavage of the propeptide, mutation in position 93 (Alabama) probably fails to bind to cell membranes, mutation in position 191 (Chapel-Hill) or in position 226 (Nagoya OR Hilo) prevent cleavage of the activation peptide. Defects in F9 are the cause of thrombophilia due to factor IX defect (THPH8). A hemostatic disorder characterized by a tendency to thrombosis. Belongs to the peptidase S1 family.

Protein type: Protease; Secreted; Secreted, signal peptide; EC 3.4.21.22

Chromosomal Location of Human Ortholog: Xq27.1-q27.2

Cellular Component: endoplasmic reticulum lumen; Golgi lumen; extracellular region; plasma membrane

Molecular Function: serine-type endopeptidase activity; calcium ion binding

Biological Process: blood coagulation, extrinsic pathway; cellular protein metabolic process; post-translational protein modification; blood coagulation; proteolysis; peptidyl-glutamic acid carboxylation; blood coagulation, intrinsic pathway

Disease: Hemophilia B; Thrombophilia, X-linked, Due To Factor Ix Defect; Coumarin Resistance