Western Blot (WB)
(Western Blot: Sample: Recombinant PRKAa1, Human.)
Rabbit anti-Human Protein Kinase Polyclonal Antibody | anti-PRKAa1 antibody
Polyclonal Antibody to Protein Kinase, AMP Activated Alpha 1 (PRKAa1)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GRL YAGPEVDIWS SGVILYALLC GTLPFDDDHV PTLFKKICDG IFYTPQYLNP SVISLLKHML QVDPMKRATI KDIREHEWFK QDLPKYLFPE DPSYSSTMID DEALKEVCEK FECSEEEVLS CLYNRNHQDP LAVAYHLIID NRRIMNEAKD FYLATSPPDS FLDDHHLTRP HPERVPFLVA ETPRARHTLD ELNPQKSKHQ GVRKAKWHLG IRSQSRPNDI MAEVCRAIKQ LDYEWKVVNP YYLRVRRK
Immunohistochemistry: 5-20ug/mL;1:20-80
Immunocytochemistry: 5-20ug/mL;1:20-80
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Recombinant PRKAa1, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant PRKAa1, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Glioma Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Glioma Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Prostate Gland Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Prostate Gland Tissue.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Uniprot Description
AMPKA1: a catalytic subunit of AMP-activated protein kinase (AMPK). Acts as an energy sensor, playing a key role in regulating cellular energy metabolism. A protein kinase of the CAMKL family whose activation is regulated by the balance between ADP/AMP/ATP, and intracellular Ca(2+) levels. Acts as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-ADP and -AMP rise in response to fuel limitation and/or hypoxia. Activates energy-producing pathways and inhibits energy-consuming processes. Restores ATP levels in cells by switching off anabolic and switching on catabolic pathways. Activated primarily by rising ADP levels and not, as previously thought, solely by AMP. AMPK resembles an adenylate charge regulatory system in which anabolic and catabolic pathways are regulated by adenine nucleotide ratios. Acts via direct phosphorylation of a number of metabolic enzymes and transcription regulators. Activated by at least two distinct upstream kinases: the tumor suppressor LKB1 and CaMKK2. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. AMPK is a heterotrimer of an alpha catalytic subunit (AMPKA1 or -2), a beta (AMPKB1 or -2) and a gamma non-catalytic subunit (AMPKG1, -2 or -3). Different possible combinations of subunits give rise to 12 different holoenzymes. Binding of ADP or AMP to non-catalytic gamma subunit (PRKAG1, -2 or -3) results in allosteric activation. AMPK is activated by antihyperglycemic drug metformin, a drug prescribed to patients with type 2 diabetes: in vivo, metformin seems to mainly inhibit liver gluconeogenesis. However, metformin can be used to activate AMPK in muscle and other cells in culture or ex vivo. Selectively inhibited by compound C (6-[4-(2-Piperidin-1-yl-ethoxy)-phenyl)]-3-pyridin-4-yl-pyyrazolo[1,5-a] pyrimidine. Activated by resveratrol, a natural polyphenol present in red wine, and S17834, a synthetic polyphenol. Two isoforms of the human protein are produced by alternative splicing.
Protein type: AMPK subfamily; Autophagy; CAMK group; CAMKL family; EC 2.7.11.1; EC 2.7.11.26; EC 2.7.11.27; EC 2.7.11.31; Kinase, protein; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 5p13.1
Cellular Component: apical plasma membrane; cytoplasm; cytosol; intracellular; nuclear speck; nucleoplasm; nucleotide-activated protein kinase complex; nucleus
Molecular Function: [acetyl-CoA carboxylase] kinase activity; [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity; AMP-activated protein kinase activity; ATP binding; cAMP-dependent protein kinase activity; chromatin binding; histone serine kinase activity; kinase binding; metal ion binding; protein binding; protein C-terminus binding; protein kinase activity; tau-protein kinase activity
Biological Process: activation of MAPK activity; CAMKK-AMPK signaling cascade; cell cycle arrest; cellular response to ethanol; cellular response to glucose starvation; cellular response to hydrogen peroxide; cellular response to hypoxia; cellular response to nutrient levels; cellular response to organonitrogen compound; cellular response to prostaglandin E stimulus; cholesterol biosynthetic process; cold acclimation; fatty acid biosynthetic process; fatty acid homeostasis; fatty acid oxidation; glucose homeostasis; glucose metabolic process; lipid biosynthetic process; macroautophagy; negative regulation of apoptosis; negative regulation of glucose import in response to insulin stimulus; negative regulation of glucosylceramide biosynthetic process; negative regulation of lipid catabolic process; negative regulation of TOR signaling; positive regulation of autophagy; positive regulation of cell proliferation; positive regulation of cholesterol biosynthetic process; positive regulation of gene expression; positive regulation of glycolysis; positive regulation of skeletal muscle tissue development; protein amino acid phosphorylation; protein heterooligomerization; regulation of circadian rhythm; regulation of energy homeostasis; regulation of macroautophagy; regulation of peptidyl-serine phosphorylation; regulation of transcription, DNA-templated; response to activity; response to caffeine; response to camptothecin; response to gamma radiation; response to hypoxia; response to UV; rhythmic process; signal transduction; transcription, DNA-dependent; Wnt signaling pathway
Research Articles on PRKAa1
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Product Notes
The PRKAa1 prkaa1 (Catalog #AAA2027174) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Protein Kinase, AMP Activated Alpha 1 (PRKAa1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Protein Kinase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/mL;1:200-800 Immunohistochemistry: 5-20ug/mL;1:20-80 Immunocytochemistry: 5-20ug/mL;1:20-80 Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the PRKAa1 prkaa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GRL YAGPEVDIWS SGVILYALLC GTLPFDDDHV PTLFKKICDG IFYTPQYLNP SVISLLKHML QVDPMKRATI KDIREHEWFK QDLPKYLFPE DPSYSSTMID DEALKEVCEK FECSEEEVLS CLYNRNHQDP LAVAYHLIID NRRIMNEAKD FYLATSPPDS FLDDHHLTRP HPERVPFLVA ETPRARHTLD ELNPQKSKHQ GVRKAKWHLG IRSQSRPNDI MAEVCRAIKQ LDYEWKVVNP YYLRVRRK. It is sometimes possible for the material contained within the vial of "Protein Kinase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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