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Typical Testing Data/Standard Curve (for reference only)

Human Coagulation Factor IX (F9) RTU ELISA Kit | F9 rtu elisa kit

Human Coagulation Factor IX (F9) ELISA Kit

Gene Names
F9; FIX; P19; PTC; HEMB; THPH8; F9 p22
Reactivity
Human
Synonyms
Coagulation Factor IX (F9); Human Coagulation Factor IX (F9) ELISA Kit; HEMB; FIX; GLA Domain; PTC; Anti Hemophilic Factor B; Christmas Factor; Plasma Thromboplastic Component; Christmas Disease; Hemophilia B; F9 rtu elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Sequence Length
461
Assay Type
Sandwich
Samples
Plasma.
Detection Range
1.25-80ng/mL
Sensitivity
0.44ng/mL
RTU ELISA Kit's Advantage
The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
Preparation and Storage
For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for F9 rtu elisa kit
Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to F9. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to F9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain F9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of F9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
47,618 Da
NCBI Official Full Name
coagulation factor IX isoform 1 preproprotein
NCBI Official Synonym Full Names
coagulation factor IX
NCBI Official Symbol
F9
NCBI Official Synonym Symbols
FIX; P19; PTC; HEMB; THPH8; F9 p22
NCBI Protein Information
coagulation factor IX
UniProt Protein Name
Coagulation factor IX
Protein Family
UniProt Gene Name
F9
UniProt Synonym Gene Names
PTC

NCBI Description

This gene encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Sep 2015]

Uniprot Description

Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca2+ ions, phospholipids, and factor VIIIa.

Research Articles on F9

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Product Notes

The Human F9 f9 (Catalog #AAA459156) is a RTU ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA459156 RTU ELISA Kit recognizes Human F9. It is sometimes possible for the material contained within the vial of "Coagulation Factor IX (F9), RTU ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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