Mouse Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1) RTU ELISA Kit | LDLRAP1 rtu elisa kit

Mouse Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1) ELISA Kit

Cat. Num. AAA4500719

• Gene Names: Ldlrap1; Arh; ARH2; Arh1; FHCB1; FHCB2; AA691260
• Reactivity: Mouse
• Synonyms: Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1); Mouse Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1) ELISA Kit; ARH1; ARH; ARH2; FHCB1; FHCB2; Autosomal recessive hypercholesterolemia protein; LDLRAP1 rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 308

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.067ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LDLRAP1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LDLRAP1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to LDLRAP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LDLRAP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LDLRAP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 160333775
• NCBI GeneID: 100017
• NCBI Accession #: NP_663529.2
• NCBI GenBank Nucleotide #: NM_145554.2
• UniProt Accession #: Q8C142; Q3TW86; Q6NWV6; Q8VDQ0
• Molecular Weight: 33,975 Da
• NCBI Official Full Name: low density lipoprotein receptor adapter protein 1
• NCBI Official Synonym Full Names: low density lipoprotein receptor adaptor protein 1
• NCBI Official Symbol: Ldlrap1
• NCBI Official Synonym Symbols: Arh; ARH2; Arh1; FHCB1; FHCB2; AA691260
• NCBI Protein Information: low density lipoprotein receptor adapter protein 1
• UniProt Protein Name: Low density lipoprotein receptor adapter protein 1
• Protein Family: Low density lipoprotein receptor adapter protein
• UniProt Gene Name: Ldlrap1

Uniprot Description

Adapter protein (clathrin-associated sorting protein (CLASP)) required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). May be required for LDL binding and internalization but not for receptor clustering in coated pits. May facilitate the endocytocis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits. May also be involved in the internalization of other LDLR family members. Binds to phosphoinositides, which regulate clathrin bud assembly at the cell surface. Required for trafficking of LRP2 to the endocytic recycling compartment which is necessary for LRP2 proteolysis, releasing a tail fragment which translocates to the nucleus and mediates transcriptional repression ().