Rabbit beta-Tubulin Polyclonal Antibody
beta-Tubulin (N14) Antibody
Reactivity
Mouse, rat, human, rabbit, Xenopus, Drosophila, zebra fish, chicken, dog, pig, cat
Applications
Western Blot
Synonyms
beta-Tubulin; Polyclonal Antibody; beta-Tubulin (N14) Antibody; anti-beta-Tubulin antibody
Host
Rabbit
Reactivity
Mouse, rat, human, rabbit, Xenopus, Drosophila, zebra fish, chicken, dog, pig, cat
Clonality
Polyclonal
Specificity
Beat-Tubulin (N14) DB111 will recognize mouse, rat, human, rabbit, Xenopus, Drosophila, zebra fish, chicken, dog, pig, and catBeta-Tubulin by western blotting and immunohistochemistry.
Form/Format
200 ug/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
Sequence Length
444
Applicable Applications for anti-beta-Tubulin antibody
Western Blot (WB)
Application Notes
Recommended Western blotting starting dilution 1:400.
Origin
Beta-Tubulin (N14) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping near the amino terminus of human beta-Tubulin.
Immunogen
A synthetic peptide mapping near the amino terminus of human beta-Tubulin.
Competition Studies
A blocking peptide is also available, MBS440099, for use in competition studies. Each vial contains 100 ug of peptide in 0.5 ml PBS with 0.1% sodium azide and 100 ug BSA
Preparation and Storage
Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.
Related Product Information for anti-beta-Tubulin antibody
The well-studied members of the Tubulin family of proteins, alpha, beta and gamma have been joined by the newest members delta, epsilon, zeta and eta (1). Microtubule function relies upon alpha-, beta-, and gamma-tubulin. Alpha and beta-Tubulin dimerize to form the microtubule filament, while gamma-tubulin combines with other proteins to form the gammasome that is essential for nucleating microtubule filaments at the centrosome (2). Once formed the microtubules function in chromosome segregation, cell shape maintenance, transport, motility and organelle distribution (3). Drugs (taxol, vinca alkaloids) that can affect the Tubulin-microtubule equilibrium have proven to be effective anticancer drugs.
References
1.) Zupanc MM, Wellbrock UM, Zupanc GK. 2006. Proteome analysis identifies novel protein candidates involved in regeneration of the cerebellum of teleost fish. Proteomics Jan;6(2):677-96.
1. Dutcher SK. 2001. The Tubulin fraternity: alpha to eta. Current Opinion Cell Biol. 13(1): 49-54. 2. Leask A, Stearns T. 1998. Expression of amino- and carboxyl-terminal gamma- and alpha-tubulin mutants in cultured epithelial cells. J. Biol. Chem. 273(5): 2661-2668. 3. Hadfield JA, Ducki S, Hirst N, McCown AT. 2003. Tubulin and microtubules as targets for anticancer drugs. Prog. Cell Cycle Res. 5:309-325.
1. Dutcher SK. 2001. The Tubulin fraternity: alpha to eta. Current Opinion Cell Biol. 13(1): 49-54. 2. Leask A, Stearns T. 1998. Expression of amino- and carboxyl-terminal gamma- and alpha-tubulin mutants in cultured epithelial cells. J. Biol. Chem. 273(5): 2661-2668. 3. Hadfield JA, Ducki S, Hirst N, McCown AT. 2003. Tubulin and microtubules as targets for anticancer drugs. Prog. Cell Cycle Res. 5:309-325.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
49,851 Da
NCBI Official Full Name
beta-tubulin
UniProt Protein Name
Tubulin beta chain
UniProt Entry Name
TBB_ACHKL
Uniprot Description
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
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Product Notes
The beta-Tubulin (Catalog #AAA440099) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The beta-Tubulin (N14) Antibody reacts with Mouse, rat, human, rabbit, Xenopus, Drosophila, zebra fish, chicken, dog, pig, cat and may cross-react with other species as described in the data sheet. AAA Biotech's beta-Tubulin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Recommended Western blotting starting dilution 1:400. Researchers should empirically determine the suitability of the beta-Tubulin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta-Tubulin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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