Rabbit ERK 2 Polyclonal Antibody | anti-ERK 2 antibody

ERK 2 (C14) Antibody

Cat. Num. AAA440020

• Reactivity: Mouse, rat, human
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry
• Synonyms: ERK 2; Polyclonal Antibody; ERK 2 (C14) Antibody; anti-ERK 2 antibody

• Host: Rabbit
• Reactivity: Mouse, rat, human
• Clonality: Polyclonal
• Specificity: Mouse, rat and human ERK 2 p42 and to a lesser extent ERK 1 p44
• Form/Format: 200 ug/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
• Concentration: 0.200 mg/ml (varies by lot)

• Applicable Applications for anti-ERK 2 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: ERK2 (C14) DB020 reacts with ERK2 p42 and to a lesser extent ERK1 p44 of mouse, rat and human origin by western blotting, immunoprecipitation, and immunohistochemistry. Western blotting starting dilution: 1:400.
• Origin: ERK2 (C14) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping to the carboxy terminus of rat ERK2.
• Immunogen: Synthetic peptide mapping to the carboxy terminus of rat ERK2.
• Preparation and Storage: Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.

Western Blot (WB)

Related Product Information for anti-ERK 2 antibody

The mitogen-activated protein kinases (MAPK) consist of several subgroups, including the ERK, JNK, and p38 kinases. The members of this MAPK family are regulated by many different extracellular cues ranging from cytokines, growth factors, and neuropeptides (1). These stimuli activate cell surface receptors to stresses such as cold, heat, osmolarity changes and irradiation. The pathways regulated by the MAPKs control a broad array of cellular responses ranging from survival, cell proliferation, and apoptosis (1,2). The MAPKs family is also characterized by their requirement for dual phosphorylation at a conserved threonine and tyrosine residue for enzymatic activation and both must be phosphorylated for full enzymatic activation (3). The closely related ERK1 (44 kDa) and ERK2 (42 kDa) kinases are characterized by their requirement for dual phosphorylation at a conserved T-E-Y motif (4,5). While JNK1 is activated by dual phosphorylation at a T-P-Y motif and p38 is also activated by dual phosphorylation at a T-G-Y motif (6,7).

References

1. Fanger GR. 1999. Regulation of the MAKP family members: role of the subcellular localization and architectural organization. Histol Histopathol 14(3):887-894. 2. Kyriakis JM. 1999. Making the connection: coupling of stress-activated ERK/MAPK (extracellular-signal-regulated kinase/mitogen-activated protein kinase) core signaling modules to extracellular stimuli and biological responses. Biochem Soc Symp 64:29-48.3. Prowse CN, Lew J. 2001. Mechanism of activation of ERK2 by dual phosphorylation. J Biol Chem 276(1):99-103.4. Kyriakis JM, Banerjee P, Nikolakaki E, Dai T, Rubie EA, Ahmad MF, Avruch J, Woodgett JR. 1994. The stress-activated protein kinase subfamily of c-Jun kinases. Nature 369(6476):156-160. 5. Cha H, Shapiro P. 2001. Tyrosine-phosphorylated extracellular signal- -regulated kinase associates with Golgi complex during G2/M phase of the cell cycle: evidence for regulation of Golgi structure. J Cell Biol 153(7):1355-1367.6. Chan ED, Winston BW, Jarpe MB, Wynes MW, Riches DW. 1997. Preferential activation of the p46 isoform of JNK/SAPK in mouse macrophages by TNF alpha. Proc Natl Acad Sci USA 94(24):13169-13174.7. Nishida E, Gotoh Y. 1993. The MAP kinase cascade is essential for diverse signal transduction pathways. Trends Biochem Sci 18:128-131.