Western Blot (WB)
Rabbit EGFR Polyclonal Antibody | anti-EGFR antibody
EGFR (I12) Antibody
Reactivity
Mouse, rat, human
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry
Synonyms
EGFR; Polyclonal Antibody; EGFR (I12) Antibody; anti-EGFR antibody
Host
Rabbit
Reactivity
Mouse, rat, human
Clonality
Polyclonal
Specificity
Mouse, rat, and human EGFR
Form/Format
200 ug/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
Concentration
0.200 mg/ml (varies by lot)
Sequence Length
10
Applicable Applications for anti-EGFR antibody
Western Blot (WB), Immunopreciptation (IP), Immunohistochemistry (IHC) Paraffin
Application Notes
Western blot analysis of EGFR expression in A431 whole cell lysates.;
Origin
EGFR (I12) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping to a sequence near the carboxy terminus of human EGFR.
Immunogen
Synthetic peptide mapping to a sequence near the carboxy terminus of human EGFR.
Preparation and Storage
Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.
Western Blot (WB)
Western Blot (WB)
Related Product Information for anti-EGFR antibody
The EFGR family is a group of four structurally similar tyrosine kinases (EGFR, HER2/neu, ErbB-3, and ErbB-4) that dimerize on binding with a number of ligands, including EGF and transforming growth factor alpha (1&2). The 170 kDa epidermal growth factor receptor binds EGF which activates a tyrosine-specific protein kinase activity intrinsic to the receptor (3&4). Epidermal growth factor receptor overexpression is pronounced in virtually all squamous carcinomas and is also found in > or = 65% of large cell and adenocarcinomas. It is not expressed in situ by small cell lung carcinoma. Overexpression of EGFR is one of the earliest and most consistent abnormalities in bronchial epithelium of high-risk smokers (1).
References
1. Franklin WA, Veve R, Hirsch FR, Helfrich BA, Bunn PA Jr. Epidermal growth factor receptor family in lung cancer and premalignancy. Semin Oncol 2002 Feb;29(1 Suppl 4):3-14.2. Yarden Y. The EGFR family and its ligands in human cancer, signalling mechanisms and therapeutic opportunities. Eur J Cancer 2001 Sep: 37 Suppl 4: S3-8.3. Reynolds F.H. Jr., Todaro G.J., Fryling C., and Stephenson J.R. Human transforming growth factors induces tyrosine phosphorylation of EGR receptors. Nature 1981 292: 259-262.4. Chen W.S., Lazar C.S., Poenie M, Tsien R.Y., Gill G.N., Rosenfeld M.G. Requirement for intrinsic protein tyrosine kinase in the immediate and late actions of the EGF receptor. Nature 1987 328: 820-823.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
1,020 Da
NCBI Official Full Name
epidermal growth factor receptor, partial
UniProt Protein Name
Epidermal growth factor receptor
Protein Family
UniProt Gene Name
c-erbB proto-oncogene
UniProt Entry Name
Q9P2Z9_HUMAN
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Product Notes
The EGFR c-erbb proto-oncogene (Catalog #AAA440047) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EGFR (I12) Antibody reacts with Mouse, rat, human and may cross-react with other species as described in the data sheet. AAA Biotech's EGFR can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunopreciptation (IP), Immunohistochemistry (IHC) Paraffin. Western blot analysis of EGFR expression in A431 whole cell lysates.;. Researchers should empirically determine the suitability of the EGFR c-erbb proto-oncogene for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EGFR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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