Western Blot (WB)
((0.3ug/ml) staining of U937 (A) and Human Spleen (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat CAPG Polyclonal Antibody | anti-CAPG antibody
Goat anti-CAPG Antibody
Western Blot: Approx 38kDa band observed in lysates of cell line U937 and of Human Spleen (calculated MW of 38.5kDa according to NP_001738.2). In Peripheral Blood Lymphocytes, the observed band runs as ~40kDa. Recommended concentration: 0.3-1ug/ml.
Western Blot (WB)
((0.3ug/ml) staining of U937 (A) and Human Spleen (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of U937 (A) and Human Spleen (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Periheral Blood Lymphocytes ( lane A), lane B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Periheral Blood Lymphocytes ( lane A), lane B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the gelsolin/villin family of actin-regulatory proteins. The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jan 2012]
Uniprot Description
CAPG: Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA. Belongs to the villin/gelsolin family.
Protein type: Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 2p11.2
Cellular Component: centriole; cytoplasm; F-actin capping protein complex; melanosome; nucleolus; nucleoplasm; nucleus
Molecular Function: actin binding; protein binding; protein complex binding; protein domain specific binding
Biological Process: barbed-end actin filament capping; extracellular matrix disassembly; protein complex assembly
Research Articles on CAPG
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Product Notes
The CAPG capg (Catalog #AAA423299) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CAPG Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's CAPG can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx 38kDa band observed in lysates of cell line U937 and of Human Spleen (calculated MW of 38.5kDa according to NP_001738.2). In Peripheral Blood Lymphocytes, the observed band runs as ~40kDa. Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the CAPG capg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: NIRATERALN WDS. It is sometimes possible for the material contained within the vial of "CAPG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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