Western Blot (WB)
(Western blot analysis of APC expression in HeLa (A), A431 (B), NIH3T3 (C), H9C2 (D) whole cell lysates.)
Rabbit APC Polyclonal Antibody | anti-APC antibody
Anti-APC Antibody
Western Blot (WB)
(Western blot analysis of APC expression in HeLa (A), A431 (B), NIH3T3 (C), H9C2 (D) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of APC expression in HeLa (A), A431 (B), NIH3T3 (C), H9C2 (D) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of APC staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of APC staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq, Jul 2008]
Uniprot Description
APC: a tumor suppressor. Defects in APC are a cause of familial adenomatous polyposis (FAP). Promotes rapid degradation of CTNNB1 and participates in Wnt signaling. Activity is correlated with its phosphorylation state. Two splice-variant isoforms have been described.
Protein type: Tumor suppressor; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 5q21-q22
Cellular Component: beta-catenin destruction complex; catenin complex; cell cortex; cell soma; cell-cell adherens junction; centrosome; cytoplasm; cytoplasmic microtubule; cytosol; dendrite; kinetochore; lamellipodium; lateral plasma membrane; nuclear membrane; nucleoplasm; nucleus; plasma membrane; synapse; tight junction
Molecular Function: beta-catenin binding; cadherin binding; gamma-catenin binding; microtubule binding; microtubule plus-end binding; protein binding; protein kinase binding; protein kinase regulator activity
Biological Process: anterior/posterior pattern formation; apoptosis; axis specification; axonogenesis; cell adhesion; cell cycle arrest; cell fate specification; cell migration; cell proliferation; cell structure disassembly during apoptosis; chromosome organization and biogenesis; cytokinesis after mitosis; cytoplasmic microtubule organization and biogenesis; dorsal/ventral pattern formation; establishment and/or maintenance of cell polarity; hair follicle development; kidney development; mitotic cell cycle spindle assembly checkpoint; mitotic metaphase/anaphase transition; muscle maintenance; negative regulation of cell proliferation; negative regulation of cyclin-dependent protein kinase activity; negative regulation of MAPKKK cascade; negative regulation of microtubule depolymerization; negative regulation of odontogenesis; pancreas development; pattern specification process; positive regulation of apoptosis; positive regulation of cell adhesion; positive regulation of cell division; positive regulation of cell migration; positive regulation of epithelial cell differentiation; positive regulation of microtubule polymerization; positive regulation of protein catabolic process; positive regulation of pseudopodium formation; programmed cell death; protein complex assembly; proximal/distal pattern formation; regulation of attachment of spindle microtubules to kinetochore; regulation of cell differentiation; regulation of microtubule-based process; regulation of osteoblast differentiation; regulation of osteoclast differentiation; response to DNA damage stimulus; response to drug; retina development in camera-type eye; somatic stem cell maintenance; T cell differentiation in the thymus; thymus development; Wnt receptor signaling pathway through beta-catenin
Disease: Colorectal Cancer; Desmoid Disease, Hereditary; Familial Adenomatous Polyposis 1; Gastric Cancer; Hepatocellular Carcinoma
Research Articles on APC
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Product Notes
The APC apc (Catalog #AAA8242047) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-APC Antibody reacts with Human, Mouse, Rat, Monkey, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's APC can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200). Researchers should empirically determine the suitability of the APC apc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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