Western Blot (WB)
(Western blot analysis of anti-AARS Pab in K562 cell line lysates (35ug/lane).AARS (arrow) was detected using the purified Pab.)
Rabbit anti-Human tRNA Synthetase, Alanyl Polyclonal Antibody | anti-AT3G16565 antibody
tRNA Synthetase, Alanyl, NT (AlaRS, Alanyl-tRNA Synthetase, Cytoplasmic, Alanine-tRNA Ligase, AlaRS, Renal Carcinoma Antigen NY-REN-42, AARS)
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
PurifiedPurified by ammonium sulfate precipitation.
Synonyms
tRNA Synthetase; Alanyl; Polyclonal Antibody; NT (AlaRS; Alanyl-tRNA Synthetase; Cytoplasmic; Alanine-tRNA Ligase; AlaRS; Renal Carcinoma Antigen NY-REN-42; AARS); Anti -tRNA Synthetase; anti-AT3G16565 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human AARS.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Purified by ammonium sulfate precipitation.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Applicable Applications for anti-AT3G16565 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:10-1:50
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:10-1:50
Immunogen
KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa68-98 from the N-terminal region of human AARS.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of anti-AARS Pab in K562 cell line lysates (35ug/lane).AARS (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-AARS Pab in K562 cell line lysates (35ug/lane).AARS (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AARS antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AARS antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Related Product Information for anti-AT3G16565 antibody
The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases,of the class II enzymes.Class II tRNA synthases evolved early in evolution and are highly conserved.This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein.tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons.They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure.
Product Categories/Family for anti-AT3G16565 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
threonyl and alanyl tRNA synthetase second additional domain-containing protein
NCBI Official Symbol
AT3G16565
NCBI Protein Information
threonyl and alanyl tRNA synthetase second additional domain-containing protein
Similar Products
Product Notes
The AT3G16565 (Catalog #AAA628242) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The tRNA Synthetase, Alanyl, NT (AlaRS, Alanyl-tRNA Synthetase, Cytoplasmic, Alanine-tRNA Ligase, AlaRS, Renal Carcinoma Antigen NY-REN-42, AARS) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's tRNA Synthetase, Alanyl can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Dilution: ELISA: 1:1,000 Western Blot: 1:50-1:100 Immunohistochemistry: 1:10-1:50. Researchers should empirically determine the suitability of the AT3G16565 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "tRNA Synthetase, Alanyl, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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