Western Blot (WB)
((1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Ide Polyclonal Antibody | anti-Ide antibody
Goat anti-Ide (mouse) Antibody
Western Blot: Approx 110kDa band observed in Mouse Brain and Rat Brain lysates (calculated MW of 118kDa according to NP_112419.2). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunising peptide.
Western Blot (WB)
((1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
Uniprot Description
IDE: Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia. Belongs to the peptidase M16 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.4.24.56; Protease; Nuclear receptor co-regulator; Cell surface
Cellular Component: cell surface; cytoplasm; cytosol; extracellular region; extracellular space; membrane; mitochondrion; nucleoplasm; nucleus; peroxisomal matrix; peroxisome; plasma membrane
Molecular Function: ATP binding; ATPase activity; beta-amyloid binding; beta-endorphin binding; catalytic activity; glycoprotein binding; hydrolase activity; insulin binding; metal ion binding; metalloendopeptidase activity; metallopeptidase activity; nucleotide binding; peptidase activity; peptide binding; peptide hormone binding; protein homodimerization activity; receptor binding; ubiquitin binding; zinc ion binding
Biological Process: beta-amyloid metabolic process; cellular protein catabolic process; determination of adult life span; hormone catabolic process; metabolic process; negative regulation of proteolysis; peptide catabolic process; positive regulation of protein oligomerization; protein heterooligomerization; protein homooligomerization; protein homotetramerization; protein processing; proteolysis; proteolysis involved in cellular protein catabolic process
Research Articles on Ide
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Product Notes
The Ide ide (Catalog #AAA421113) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Ide (mouse) Antibody reacts with Tested: Mouse, Rat; Expected from sequence similarity: Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Ide can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 110kDa band observed in Mouse Brain and Rat Brain lysates (calculated MW of 118kDa according to NP_112419.2). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunising peptide. Researchers should empirically determine the suitability of the Ide ide for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QQYNYDRDNI E. It is sometimes possible for the material contained within the vial of "Ide, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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