Western Blot (WB)
((0.5ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat ASRGL1/ALP Polyclonal Antibody | anti-ASRGL1 antibody
Goat anti-ASRGL1/ALP Antibody
Gene Names
ASRGL1; ALP; ALP1; CRASH
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
ASRGL1/ALP; Polyclonal Antibody; Goat anti-ASRGL1/ALP Antibody; ASRGL1; ALP; asparaginase like 1; ALP1; FLJ22316; asparaginase-like 1 protein; ASRGL1 antibody; ALP antibody; asparaginase like 1 antibody; ALP1 antibody; FLJ22316 antibody; asparaginase-like 1 protein antibody; ASRGL1 / ALP; anti-ASRGL1 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
EKHEKGAQKTDCQ
Sequence Length
308
Applicable Applications for anti-ASRGL1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 34kDa band observed in Human Tosil and lysteof cell line Hela lysates (calculated MW of 32.1kDa according to NP_001077395.1). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunizing peptide.
Western Blot: Approx 34kDa band observed in Human Tosil and lysteof cell line Hela lysates (calculated MW of 32.1kDa according to NP_001077395.1). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunizing peptide.
Immunogen
Peptide with sequence EKHEKGAQKTDCQ, from the internal region of the protein sequence according to NP_001077395.1; NP_079356.3.
Epitope
Internal region
Note
Both reported variants represent identical protein (NP_001077395.1 and NP_079356.3).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.5ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.5ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,981 Da
NCBI Official Full Name
isoaspartyl peptidase/L-asparaginase
NCBI Official Synonym Full Names
asparaginase like 1
NCBI Official Symbol
ASRGL1
NCBI Official Synonym Symbols
ALP; ALP1; CRASH
NCBI Protein Information
isoaspartyl peptidase/L-asparaginase
UniProt Protein Name
Isoaspartyl peptidase/L-asparaginase
Protein Family
UniProt Gene Name
ASRGL1
UniProt Synonym Gene Names
ALP; CRASH
UniProt Entry Name
ASGL1_HUMAN
Uniprot Description
ASRGL1: Acts in asparagine catabolism. May be involved in astroglial production of L-aspartate, which can act as an excitatory neurotransmitter in some brain regions. Belongs to the Ntn-hydrolase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.5.1.1; Hydrolase; EC 3.4.19.5; Other Amino Acids Metabolism - cyanoamino acid; Energy Metabolism - nitrogen; Amino Acid Metabolism - alanine, aspartate and glutamate
Chromosomal Location of Human Ortholog: 11q12.3
Cellular Component: cytoplasm; cytosol; nucleus
Molecular Function: asparaginase activity; beta-aspartyl-peptidase activity; N4-(beta-N-acetylglucosaminyl)-L-asparaginase activity
Biological Process: asparagine catabolic process via L-aspartate; L-phenylalanine catabolic process; proteolysis
Research Articles on ASRGL1
Similar Products
Product Notes
The ASRGL1 asrgl1 (Catalog #AAA421566) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ASRGL1/ALP Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's ASRGL1/ALP can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 34kDa band observed in Human Tosil and lysteof cell line Hela lysates (calculated MW of 32.1kDa according to NP_001077395.1). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the ASRGL1 asrgl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: EKHEKGAQKT DCQ. It is sometimes possible for the material contained within the vial of "ASRGL1/ALP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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