Western Blot (WB)
(MBS423363 (0.3µg/ml) staining of MCF7 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.)
Goat HNRNPA2B1 Polyclonal Antibody | anti-HNRNPA2B1 antibody
Goat anti-HNRNPA2B1 Antibody
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Western blot: Approx 37kDa band observed in lysates of cell line MCF7 and approx. 35kDa in lysates of cell line NIH3T3 and nuclear NIH3T3 (calculated MW of 36.0kDa according to NP_002128.1). An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunising peptide. Recommended concentration: 0.01-0.3µg/ml. Primary incubation 1 hour at room temperature.
IHC: In paraffin embedded Human Small Intestine shows nuclear staining. Paraffin embedded Human Testis. Recommended concentration: 5µg/ml.
Western Blot (WB)
(MBS423363 (0.3µg/ml) staining of MCF7 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.)
Western Blot (WB)
(MBS423363 (0.3µg/ml) staining of MCF7 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.)
Western Blot (WB)
(MBS423363 (0.1µg/ml) staining of NIH3T3 (A), (0.01ug/ml) nuclear NIH3T3 (B) lysate, + Peptide (C) (35µg protein in RIPA buffer). Detected by chemiluminescence.)
Western Blot (WB)
(MBS423363 (0.1µg/ml) staining of NIH3T3 (A), (0.01ug/ml) nuclear NIH3T3 (B) lysate, + Peptide (C) (35µg protein in RIPA buffer). Detected by chemiluminescence.)
Immunohistochemistry (IHC)
(MBS423363 (5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
(MBS423363 (5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
(MBS423363 (5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
(MBS423363 (5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
hnRNP A2/B1: Involved with pre-mRNA processing. Forms complexes (ribonucleosomes) with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleus. Identified in the spliceosome C complex. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with IGF2BP1. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Spliceosome; RNA-binding; RNA splicing
Chromosomal Location of Human Ortholog: 7p15
Cellular Component: cytoplasm; membrane; nucleoplasm; nucleus; ribonucleoprotein complex; spliceosome
Molecular Function: miRNA binding; mRNA 3'-UTR binding; nucleotide binding; protein binding; RNA binding; single-stranded telomeric DNA binding
Biological Process: gene expression; mRNA export from nucleus; mRNA processing; negative regulation of nuclear mRNA splicing, via spliceosome; negative regulation of transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; primary microRNA processing; RNA transport
Disease: Inclusion Body Myopathy With Early-onset Paget Disease With Or Without Frontotemporal Dementia 2
Research Articles on HNRNPA2B1
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Product Notes
The HNRNPA2B1 hnrnpa2b1 (Catalog #AAA423363) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-HNRNPA2B1 Antibody reacts with Tested: Human, Mouse Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's HNRNPA2B1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western blot: Approx 37kDa band observed in lysates of cell line MCF7 and approx. 35kDa in lysates of cell line NIH3T3 and nuclear NIH3T3 (calculated MW of 36.0kDa according to NP_002128.1). An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunising peptide. Recommended concentration: 0.01-0.3µg/ml. Primary incubation 1 hour at room temperature. IHC: In paraffin embedded Human Small Intestine shows nuclear staining. Paraffin embedded Human Testis. Recommended concentration: 5µg/ml. Researchers should empirically determine the suitability of the HNRNPA2B1 hnrnpa2b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DTIEIITDRQ S. It is sometimes possible for the material contained within the vial of "HNRNPA2B1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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