Goat EBPL41L5 Polyclonal Antibody | anti-EPB41L5 antibody

Goat anti-EBPL41L5 Antibody

Cat. Num. AAA421370

• Gene Names: EPB41L5; YRT; BE37; YMO1
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: EBPL41L5; Polyclonal Antibody; Goat anti-EBPL41L5 Antibody; EPB41L5; erythrocyte membrane protein band 4.1 like 5; BE37; FLJ12957; KIAA1548; EPB41L5 antibody; erythrocyte membrane protein band 4.1 like 5 antibody; BE37 antibody; FLJ12957 antibody; KIAA1548 antibody; anti-EPB41L5 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: ENLPQSPGTDQHD
• Sequence Length: 733

• Applicable Applications for anti-EPB41L5 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 8000.; Western Blot: Approx 80kDa band observed in Human Brain (Hippocampus lysates (calculated MW of 81.9kDa according to NP_065960.2). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 140kDa. This band was successfully blocked by incubation with the immunizing peptide.
• Immunogen: Peptide with sequence C-ENLPQSPGTDQHD, from the internal region of the protein sequence according to NP_065960.2.
• Epitope: Internal region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((1ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 40254949
• NCBI GeneID: 57669
• NCBI Accession #: NP_065960.2
• NCBI GenBank Nucleotide #: NM_020909.3
• Molecular Weight: 77,520 Da
• NCBI Official Full Name: band 4.1-like protein 5 isoform 1
• NCBI Official Synonym Full Names: erythrocyte membrane protein band 4.1 like 5
• NCBI Official Symbol: EPB41L5
• NCBI Official Synonym Symbols: YRT; BE37; YMO1
• NCBI Protein Information: band 4.1-like protein 5
• UniProt Protein Name: Band 4.1-like protein 5
• Protein Family: Band 4.1-like protein
• UniProt Gene Name: EPB41L5
• UniProt Synonym Gene Names: KIAA1548
• UniProt Entry Name: E41L5_HUMAN

Uniprot Description

EPB41L5: May contribute to the correct positioning of tight junctions during the establishment of polarity in epithelial cells. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 2q14.2

Cellular Component: cytoplasm; cytoskeleton; extrinsic to membrane; focal adhesion; nucleus; plasma membrane

Molecular Function: cytoskeletal protein binding; protein domain specific binding

Biological Process: actomyosin structure organization and biogenesis; axial mesoderm morphogenesis; ectoderm development; embryonic foregut morphogenesis; endoderm development; epithelial to mesenchymal transition; in utero embryonic development; mesoderm migration; negative regulation of cell-cell adhesion; negative regulation of protein binding; neural plate morphogenesis; paraxial mesoderm development; positive regulation of focal adhesion formation; positive regulation of protein binding; somite rostral/caudal axis specification; substrate-bound cell migration, cell attachment to substrate; unidimensional cell growth