Testing Data
DNA enzyme
E Coli DNA Ligase
Applications
Ligation of dsDNA with cohesive terminicDNA cloning of products from second strand cDNA synthesis experiments
Alternative to T4 DNA Ligase when blunt-end ligation is not required
For ligation of blunt-end fragments use T4 DNA Ligase
Note: Thi
Synonyms
DNA; E Coli DNA Ligase; DNA enzyme
Applicable Applications for DNA enzyme
Ligation of dsDNA with cohesive termini
cDNA cloning of products from second strand cDNA synthesis experiments
Alternative to T4 DNA Ligase when blunt-end ligation is not required
For ligation of blunt-end fragments use T4 DNA Ligase
Note: Thi
cDNA cloning of products from second strand cDNA synthesis experiments
Alternative to T4 DNA Ligase when blunt-end ligation is not required
For ligation of blunt-end fragments use T4 DNA Ligase
Note: Thi
Storage Buffer
50 mM Tris-HCl (pH 7.5), 1 mM EDTA, 1 mM DTT, 200 mM NaCl, and 50% (v/v) Glycerol.
Notes
One unit is defined as the amount of E Coli DNA Ligase that is required to give 50% ligation of HindIII-digested lambda DNA in a total reaction volume of 20 ul in 30 minutes at 16 degree C in 1X E Coli DNA Ligase Reaction Buffer (concentration of 5 DNA termini is 0.12 uM [300 ug/ml]).
Preparation and Storage
Store all components at -20 degree C.
Testing Data
Testing Data
Related Product Information for DNA enzyme
E Coli DNA Ligase is an NAD+-dependent enzyme that catalyzes the formation of a phosphodiester bond between cohesive 3-hydroxyl and 5-phosphoryl termini of double-stranded DNA (dsDNA). This enzyme is also active on nicked DNA but is not effective for the formation of DNA-RNA or RNA-RNA hybrids.
Similar Products
Product Notes
The DNA (Catalog #AAA4156420) is an Enzyme and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DNA can be used in a range of immunoassay formats including, but not limited to, Ligation of dsDNA with cohesive termini cDNA cloning of products from second strand cDNA synthesis experiments Alternative to T4 DNA Ligase when blunt-end ligation is not required For ligation of blunt-end fragments use T4 DNA Ligase Note: Thi. Researchers should empirically determine the suitability of the DNA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
