Retinol binding protein 4 (RBP4) Recombinant Protein | RBP4 recombinant protein

Retinol binding protein 4 (RBP4): Human, (Recombinant Protein)

Cat. Num. AAA398074

• Gene Names: RBP4A; RBP; RBP4
• Applications: ELISA, Western Blot
• Purity: >95%; SDS PAGE to determine purity of the protein
• Synonyms: Retinol binding protein 4 (RBP4); Retinol binding protein 4 (RBP4): Human; (Recombinant Protein); Rentinol binding protein 4; Recombinant Human Retinol Binding Protein 4; RBP4 recombinant protein

• Host: E Coli
• Purity/Purification: >95%; SDS PAGE to determine purity of the protein
• Form/Format: Lyophilized in 1mg/mL in PBS
• Sequence: MSYYHHHHHH DYDIPTTENL YFQGAMGSER DCRVSSFRVK ENFDKARFSG TWYAMAKKDP EGLFLQDNIV AEFSVDETGQ MSATAKGRVR LLNNWDVCAD MVGTFTDTED PAKFKMKYWG VASFLQKGND DHWIVDTDYD TYAVQYSCRL LNLDGTCADS YSFVFSRDPN GLPPEAQKIV RQRQEELCLA RQYRLLIVHNG YCKGRSERNL L
• Sequence Length: 196

• Applicable Applications for RBP4 recombinant protein: ELISA (EIA), Western Blot (WB)
• Species: Human
• Tag: His Tag
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 1mg/mL and let the lyophilized pellet dissolve completely.
• Quality Control Test: BCA to determine quantity of the protein.
• Preparation and Storage: Store lyophilized protein at -20 degree C. Aliquot reconstituted protein and store at -80 degree C. Avoid repeated freezing /thawing cycles.

SDS-Page

Related Product Information for RBP4 recombinant protein

Retinol binding protein 4 (RBP4), originally known as a specific transport of retinol in blood, is also a novel inflammatory and insulin resistance marker. Serum RBP4 levels are elevated in insulin-resistant mice and humans with obesity and type 2 diabetes. Animal experiments found that increased secretion of RBP4 might reduces insulin-dependent glucose uptake by muscle tissue by reducing the activity of PI(3)K (phosphoinositide 3-kinase), and increased hepatic glucose output by increasing the expression of the enzyme PEPCK2. Studies suggested that elevated serum RBP4 was associated with components of the metabolic syndrome, including increased body-mass index, waist-to-hip ratio, serum triglyceride levels, and systolic blood pressure and decreased high- density lipoprotein cholesterol levels. Studies both in human and animal suggested that serum levels of RBP4 plays a key role in the link between obesity,insulin resistant and diabetes. Animal experiments found that increased secretion ofRBP4 might reduces insulin-dependent glucose uptake by muscle tissue by reducingthe activity of PI(3)K (phosphoinositide 3-kinase), and increased hepatic glucoseoutput by increasing the expression of the enzyme PEPCK. Furthermore, circulating RBP4 concentrations were associated with subclinical cardiovascular disease, which imply that RBP4 could be involved in the development of atherosclerosis. However, whether serumRBP4 could be a biomarker of type 2 diabetes risk still remain unclear.
Total 211 AA. Mw: 24.4 kDa (calculated). N-terminal His-tag and TEV cleavage site, 28 extra AA.

References

1.The metabolic role of retinol binding protein 4: an update. Christou GA, Tselepis AD, Kiortsis DN. Horm Metab Res. 2012 44(1):6-14.
2. RBP4: a controversial adipokine. Kotnik P, Fischer-Posovszky P, Wabitsch M. Eur J Endocrinol. 2011 Nov;165(5):703-11.

NCBI and Uniprot Product Information

• NCBI GI #: 45382541
• NCBI GeneID: 396166
• NCBI GenBank Nucleotide #: NP_990569.1
• UniProt Accession #: P41263
• Molecular Weight: 24.4kDa
• NCBI Official Full Name: retinol-binding protein 4
• NCBI Official Synonym Full Names: retinol binding protein 4 A, plasma
• NCBI Official Symbol: RBP4A
• NCBI Official Synonym Symbols: RBP; RBP4
• NCBI Protein Information: retinol-binding protein 4
• UniProt Protein Name: Retinol-binding protein 4
• Protein Family: Retinol-binding protein
• UniProt Gene Name: RBP4
• UniProt Synonym Gene Names: PRBP; RBP
• UniProt Entry Name: RET4_CHICK

Uniprot Description

Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli.