Lipocalin-2 Recombinant Protein | LCN2 recombinant protein

Lipocalin-2: Mouse (Recombinant protein)

Cat. Num. AAA398085

• Gene Names: LCN2; p25; 24p3; MSFI; NGAL
• Applications: ELISA, Western Blot
• Purity: >95%; SDS PAGE to determine purity of the protein
• Synonyms: Lipocalin-2; Lipocalin-2: Mouse (Recombinant protein); Lipocalin 2; Recombinant Mouse Lipocalin-2 (NGAL); LCN2 recombinant protein

• Host: E Coli
• Purity/Purification: >95%; SDS PAGE to determine purity of the protein
• Form/Format: Lyophilized in 1mg/mL in PBS
• Sequence: MSYYHHHHHH DYDIPTTENL YFQGAMGS QDSTQNLIPA PSLLTVPLQP DFRSDQFRGR WYVVGLAGNA VQKKTEGSFT MYSTIYELQE NNSYNTSIL VRDQDQGCRY WIRTFVPSSR AGQFTLGNMH RYPQVQSYNV AVATTDYNQF AMVFFRKTSE NKQYFKITLY GRTKELSPEL KERFTRFAKS LGLKDDNIIF SVPTDQCIDN
• Sequence Length: 198

• Applicable Applications for LCN2 recombinant protein: ELISA (EIA), Western Blot (WB)
• Species: Mouse
• Tag: His Tag
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 1mg/mL and let the lyophilized pellet dissolve completely.
• Quality Control Test: BCA to determine quantity of the protein.
• Preparation and Storage: Store lyophilized protein at -20 degree C. Aliquot reconstituted protein and store at -80 degree C. Avoid repeated freezing /thawing cycles.

SDS-Page

Related Product Information for LCN2 recombinant protein

Lipocalin-2 (LCN2), also known as neutrophil gelatinase-associated lipocalin (NGAL), 24p3, or neutrophil lipocalin (NL), is a 25-kDa secretory glycoprotein which isexpressed in liver, lung, kidney, adipocytes, activated neutrophils, and macrophages. LCN2 has been implicated in a variety of cellular processes including the innate immune response, infection, differentiation, tumorigenesis and cell survival. It appears to be up- regulated in various inflammation and infection conditions. Several reports suggest that LCN2 may represent a sensitive biomarker for various renal and lung injuries and is associated with several types of cancers, including breast cancer, ovarian, colorectal and pancreatic cancers. Furthermore, serum levels of LCN2 are correlated with obesity, insulin resistance, hyperglycemia, coronary heart disease and fatty liver disease in humans. LCN2 is also high expressed under high fat diet andobese background especially in adipose tissue and is closely associated with obesity,insulin resistance, and hyperglycemia in humans.
Total 208AA. Mw: 24.2kDa (calculated). N-terminal His-tag and TEV cleavage site, 28 extra AA.

References

1. A emerging clinical and experimental evidence for the role of lipocalin-2 in metabolic syndrome. Jang Y, Lee JH, Wang Y, Sweeney G. Clin Exp Pharmacol Physiol. 2012 Feb;39(2):194-9
2. The multifaceted roles of neutrophil gelatinase associated lipocalin (NGAL) in inflammation and cancer. Chakraborty S, Kaur S, Guha S, Batra SK. Biochim Biophys Acta. 2012 Aug;1826(1):129-69.

NCBI and Uniprot Product Information

• NCBI GI #: 21619839
• NCBI GeneID: 3934
• Molecular Weight: 24.2kDa
• NCBI Official Full Name: Lipocalin 2
• NCBI Official Synonym Full Names: lipocalin 2
• NCBI Official Symbol: LCN2
• NCBI Official Synonym Symbols: p25; 24p3; MSFI; NGAL
• NCBI Protein Information: neutrophil gelatinase-associated lipocalin
• Protein Family: Neutrophil gelatinase-associated lipocalin

NCBI Description

This gene encodes a protein that belongs to the lipocalin family. Members of this family transport small hydrophobic molecules such as lipids, steroid hormones and retinoids. The protein encoded by this gene is a neutrophil gelatinase-associated lipocalin and plays a role in innate immunity by limiting bacterial growth as a result of sequestering iron-containing siderophores. The presence of this protein in blood and urine is an early biomarker of acute kidney injury. This protein is thought to be be involved in multiple cellular processes, including maintenance of skin homeostasis, and suppression of invasiveness and metastasis. Mice lacking this gene are more susceptible to bacterial infection than wild type mice. [provided by RefSeq, Sep 2015]