Human Retinol binding protein 4 (RBP4) ELISA Kit | RBP4 elisa kit

Retinol binding protein 4 (RBP4): Human, (ELISA)

Cat. Num. AAA398049

• Gene Names: RBP4A; RBP; RBP4
• Reactivity: Human
• Synonyms: Retinol binding protein 4 (RBP4); Retinol binding protein 4 (RBP4): Human; (ELISA); Human retinol binding protein (RBP4); RBP4 elisa kit

• Reactivity: Human
• Specificity: The antibody pair used in this assay is specific to human RBP4 and does not cross-react with mouse and rat RBP4, and other cytokine or hormone molecules.
• Sequence Length: 196

• Samples: Cell culture supernates, serum, and plasma
• Assay Type: Quantitative Sandwich
• Sensitivity: 3.12 ng/ml
• Intra-assay Precision: Three samples of known concentration were tested 16 times on one plate.
• Inter-assay Precision: Three samples of known concentration were tested in 10 separate assays.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RBP4 elisa kit

Intended Uses: This Human RBP4 ELISA kit is designed for quantification of Human RBP4 in serum, plasma, and adipocyte extracts or cell culture media samples.

Principle of the Assay: This assay is a quantitative sandwich ELISA using monoclonal antibodies against human RBP4. The immunoplate is pre-coated with a monoclonal antibody specific for human RBP4 and the nonspecific binding sites are blocked. Standards and samples are pipetted into the wells and any Human RBP4 present is bound by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidase (HRP)-linked monoclonal antibody specific for human RBP4 is added to the wells. After wash step to remove any unbound reagents, an HRP substrate solution is added and colour develops in proportion to the amount of human RBP4 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human RBP4, the unknown sample concentration can be interpolated from a reference curve included in each assay.

Background/Introduction: Retinol binding protein 4(RBP4), originally known as a specific transport of retinol in blood, is also a novel inflammatory and insulin resistance marker1. Serum RBP4 levels are elevated in insulin-resistant mice and humans with obesity and type 2 diabetes2,3. Animal experiments found that increased secretion of RBP4 might reduces insulin-dependent glucose uptake by muscle tissue by reducing the activity of PI(3)K (phosphoinositide 3-kinase), and increased hepatic glucose output by increasing the expression of the enzyme PEPCK2. Studies suggested that elevated serum RBP4 was associated with components of the metabolic syndrome, including increased body-mass index, waist-to-hip ratio, serum triglyceride levels, and systolic blood pressure and decreased highdensity lipoprotein cholesterol levels4. Furthermore, circulating RBP4 concentrations were associated with subclinical cardiovascular disease, which imply that RBP4 could be involved in the development of atherosclerosis5.

References

1.The metabolic role of retinol binding protein 4: an update. Christou GA, Tselepis AD, Kiortsis DN. Horm Metab Res. 2012 44(1):6-14.
2. RBP4: a controversial adipokine. Kotnik P, Fischer-Posovszky P, Wabitsch M. Eur J Endocrinol. 2011 Nov;165(5):703-11.

NCBI and Uniprot Product Information

• NCBI GI #: 45382541
• NCBI GeneID: 396166
• NCBI GenBank Nucleotide #: NP_990569.1
• UniProt Accession #: P41263
• NCBI Official Full Name: retinol-binding protein 4
• NCBI Official Synonym Full Names: retinol binding protein 4 A, plasma
• NCBI Official Symbol: RBP4A
• NCBI Official Synonym Symbols: RBP; RBP4
• NCBI Protein Information: retinol-binding protein 4
• UniProt Protein Name: Retinol-binding protein 4
• Protein Family: Retinol-binding protein
• UniProt Gene Name: RBP4
• UniProt Synonym Gene Names: PRBP; RBP
• UniProt Entry Name: RET4_CHICK

Uniprot Description

Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli.