Human CD54 ELISA Kit | ICAM1 elisa kit

Human CD54 ELISA Kit

Cat. Num. AAA8244612

• Gene Names: ICAM1; BB2; CD54; P3.58
• Reactivity: Human
• Applications: ELISA
• Synonyms: CD54; Human CD54 ELISA Kit; Intercellular adhesion molecule 1; ICAM-1; Major group rhinovirus receptor; ICAM1 elisa kit

• Reactivity: Human
• Specificity: The Human CD54 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human CD54 proteins within the range of 62.5 pg/ml - 4000 pg/ml.
• Sequence Length: 532

• Applicable Applications for ICAM1 elisa kit: Sandwich ELISA (EIA)
• Samples: Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates.
• Sensitivity: 30 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve

Related Product Information for ICAM1 elisa kit

Principle of the Assay||The Human CD54 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD54 in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Human CD54 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD54 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD54 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD54 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin. The normal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen (LFA-1) or macrophage-1 antigen (Mac-1). ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses.

NCBI and Uniprot Product Information

• NCBI GI #: 68067956
• NCBI GeneID: 3383
• UniProt Accession #: P05362; Q5NKV7; Q96B50; B2R6M3
• Molecular Weight: 57,825 Da
• NCBI Official Full Name: Intercellular adhesion molecule 1
• NCBI Official Synonym Full Names: intercellular adhesion molecule 1
• NCBI Official Symbol: ICAM1
• NCBI Official Synonym Symbols: BB2; CD54; P3.58
• NCBI Protein Information: intercellular adhesion molecule 1
• UniProt Protein Name: Intercellular adhesion molecule 1
• Protein Family: Intercellular adhesion molecule
• UniProt Gene Name: ICAM1
• UniProt Synonym Gene Names: ICAM-1
• UniProt Entry Name: ICAM1_HUMAN

NCBI Description

This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]

Uniprot Description

ICAM1: a type I membrane protein of the immunoglobulin superfamily. Is a ligand for the leukocyte adhesion LFA-1 protein (Integrin alpha-L/beta-2) and a Rhinovirus receptor. Typically expressed on endothelial cells and cells of the immune system. ICAM1 binds to integrins of type CD11a / CD18, or CD11b / CD18. Its expression is activated by p53 in an NF-kappaB-independent manner. Induced by TNFalpha in a process that involves IKKbeta.

Protein type: Membrane protein, integral; Cell adhesion; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: cell surface; external side of plasma membrane; extracellular space; focal adhesion; immunological synapse; integral to plasma membrane; lipid raft; membrane; plasma membrane

Molecular Function: integrin binding; protein binding; receptor activity; transmembrane receptor activity; viral receptor activity

Biological Process: activation of NF-kappaB transcription factor; acute inflammatory response to antigenic stimulus; adhesion to host; cell adhesion; cell adhesion mediated by integrin; cell aging; cellular response to nutrient levels; cytokine and chemokine mediated signaling pathway; entry of virus into host cell; extracellular matrix organization and biogenesis; heterophilic cell adhesion; leukocyte adhesion; leukocyte migration; membrane to membrane docking; negative regulation of calcium ion transport; ovarian follicle development; positive regulation of actin filament polymerization; positive regulation of cellular extravasation; positive regulation of GTPase activity; positive regulation of nitric oxide biosynthetic process; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of vasoconstriction; regulation of cell adhesion; regulation of cell shape; regulation of immune response; regulation of leukocyte mediated cytotoxicity; response to amino acid stimulus; response to amphetamine; response to copper ion; response to drug; response to ethanol; response to ionizing radiation; response to organic cyclic substance; response to sulfur dioxide; sensory perception of sound; T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; T cell antigen processing and presentation; virion attachment, binding of host cell surface receptor

Disease: Malaria, Susceptibility To