Rabbit G 5-D1 Polyclonal Antibody | anti-G 5-D1 antibody

G 5-D1

Cat. Num. AAA395507

• Gene Names: GNB5; GB5
• Reactivity: Murine, Rat, Human
• Applications: Western Blot
• Purity: Ammonium Sulfate Precipitation
• Synonyms: G 5-D1; Polyclonal Antibody; anti-G 5-D1 antibody

• Host: Rabbit
• Reactivity: Murine, Rat, Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Ammonium Sulfate Precipitation
• Form/Format: Provided as solution in phosphate buffered saline with 0.08% sodium azide
• Sequence Length: 353

• Applicable Applications for anti-G 5-D1 antibody: Western Blot (WB)
• Immunogen: Unique peptide sequence derived from the D1 blade of the G 5 protein.
• Positive Control: Plasma membranes from rat cerebrum
• Preparation and Storage: Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles

Western Blot/Blotting

(Western blot analysis using G 5 antibody on 20 ng (1) and 10 ng (2) purified G 5 protein.)

Related Product Information for anti-G 5-D1 antibody

The Gbeta5 protein is a G protein subunit present predominantly in the central nervous system. This protein appears to be expressed in two forms, one found in the brain (39 kDa) and one found in the retina (44 kDa). It has also been found that Gb5 associates tightly with RGS7, a member of the superfamily of negative regulators of G protein signaling. These complexes have been localized to most regions of the brain, whereas G 1-4 exhibit no observable colocalization with RGS7, despite their widespread codistribution throughout similar neuronal networks. This antibody is specific for a peptide sequence within the D1 blade which is not found in the Gbeta?s 1-4.

Product Categories/Family for anti-G 5-D1 antibody

Polyclonal Antibody; G Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 5729852
• NCBI GeneID: 10681
• NCBI Accession #: NP_006569.1
• NCBI GenBank Nucleotide #: NM_006578.3
• UniProt Accession #: O14775; Q9HAU9; Q9UFT3
• Molecular Weight: 43,566 Da
• NCBI Official Full Name: guanine nucleotide-binding protein subunit beta-5 isoform a
• NCBI Official Synonym Full Names: guanine nucleotide binding protein (G protein), beta 5
• NCBI Official Symbol: GNB5
• NCBI Official Synonym Symbols: GB5
• NCBI Protein Information: guanine nucleotide-binding protein subunit beta-5; gbeta5; transducin beta chain 5; G protein, beta-5 subunit; G protein, beta subunit 5L; guanine nucleotide-binding protein, beta subunit 5L
• UniProt Protein Name: Guanine nucleotide-binding protein subunit beta-5
• UniProt Gene Name: GNB5
• UniProt Entry Name: GBB5_HUMAN

NCBI Description

Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Alternatively spliced transcript variants encoding different isoforms exist. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

Subunit structure: G proteins are composed of 3 units, alpha, beta and gamma. Component of the RGS9-1-Gbeta5 complex composed of RGS9 (isoform RGS9-1), Gbeta5 (GNB5) and RGS9BP

By similarity.

Tissue specificity: Expressed in multiple tissues.

Sequence similarities: Belongs to the WD repeat G protein beta family.Contains 7 WD repeats.