Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (Western Blot Analysis: L6 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-G protein Gbeta5 (1:500 dilution of this lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates G protein Gbeta5 (~40kD).)

Rabbit Guanine nucleotide binding protein, beta 5 Subunit Polyclonal Antibody | anti-Gbeta5 antibody

Guanine nucleotide binding protein, beta 5 Subunit (Gbeta5, FLJ37457, FLJ43714, GB5, Gbeta5, Guanine nucleotide-binding protein subunit beta-5, Transducin beta Chain 5) (MaxLight 490)

Gene Names
GNB5; GB5
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Purified
Synonyms
Guanine nucleotide binding protein; beta 5 Subunit; Polyclonal Antibody; beta 5 Subunit (Gbeta5; FLJ37457; FLJ43714; GB5; Gbeta5; Guanine nucleotide-binding protein subunit beta-5; Transducin beta Chain 5) (MaxLight 490); anti-Gbeta5 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes D1 area of human Gb5. Species Crossreactivity: mouse and rat.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Applicable Applications for anti-Gbeta5 antibody
Western Blot (WB)
Application Notes
WB: 1:500
Applications are based on unconjugated antibody.
Immunogen
Unique peptide sequence derived from the D1 blade of the Gb5 protein.
Conjugate
MaxLight490
Positive Control
L6 Cell Lysate, plasma membranes from rat cerebrum.
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot Analysis: L6 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-G protein Gbeta5 (1:500 dilution of this lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates G protein Gbeta5 (~40kD).)

Western Blot (WB) (Western Blot Analysis: L6 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-G protein Gbeta5 (1:500 dilution of this lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates G protein Gbeta5 (~40kD).)
Product Categories/Family for anti-Gbeta5 antibody
References
1. Liang, J.J., et al. (2000). J. Neurosci. Res. 60:58-64. 2. Cabrera, J.L., et al. (1998). Biochem Biophys Res Commun. 249:898-902. 3. Watson, A.J., et al. (1996). J. Biol. Chem. 271:28154-28160.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43,566 Da
NCBI Official Full Name
guanine nucleotide-binding protein subunit beta-5 isoform a
NCBI Official Synonym Full Names
guanine nucleotide binding protein (G protein), beta 5
NCBI Official Symbol
GNB5
NCBI Official Synonym Symbols
GB5
NCBI Protein Information
guanine nucleotide-binding protein subunit beta-5; gbeta5; transducin beta chain 5; G protein, beta-5 subunit; G protein, beta subunit 5L; guanine nucleotide-binding protein, beta subunit 5L
UniProt Protein Name
Guanine nucleotide-binding protein subunit beta-5
UniProt Gene Name
GNB5
UniProt Entry Name
GBB5_HUMAN

NCBI Description

Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Alternatively spliced transcript variants encoding different isoforms exist. [provided by RefSeq, Jul 2008]

Uniprot Description

G-beta 5: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction. Belongs to the WD repeat G protein beta family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein, heterotrimeric beta

Chromosomal Location of Human Ortholog: 15q21.2

Cellular Component: plasma membrane; nucleus

Molecular Function: GTPase activity; G-protein gamma-subunit binding; protein binding; signal transducer activity; chaperone binding

Biological Process: rhodopsin mediated signaling; phototransduction, visible light; regulation of rhodopsin mediated signaling; metabolic process; signal transduction

Research Articles on Gbeta5

Similar Products

Product Notes

The Gbeta5 gnb5 (Catalog #AAA6479282) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Guanine nucleotide binding protein, beta 5 Subunit (Gbeta5, FLJ37457, FLJ43714, GB5, Gbeta5, Guanine nucleotide-binding protein subunit beta-5, Transducin beta Chain 5) (MaxLight 490) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Guanine nucleotide binding protein, beta 5 Subunit can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Gbeta5 gnb5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Guanine nucleotide binding protein, beta 5 Subunit, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.