MePCE recombinant protein

Recombinant MePCE protein

Cat. Num. AAA389241

• Gene Names: MEPCE; BCDIN3
• Synonyms: MePCE; Recombinant MePCE protein; Methylphosphate Capping Enzyme; Bicoid-Interacting Protein 3 Homolog; BCDIN3; MePCE recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant MEPCE protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant MEPCE protein gel 10% SDS-PAGE Coomassie staining MW: 75.6 kDa Purity: >85%)

Related Product Information for MePCE recombinant protein

Short Description: Recombinant MEPCE protein was expressed in a baculovirus expression system as the full-length protein (accession number NP_062552.2) with an N-terminal DYKDDDDK-Tag. The molecular weight of the protein is 75.6 kDa.

Background: MEPCE (Methylphosphate Capping Enzyme), also called as BCDIN3 (Bicoid-Interacting Protein 3 Homolog), is a RNA methyltransferase. It's a S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5-end of 7SK snRNA, leading to stabilize it. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, MEPCE/BCDIN3, SART3 proteins and 7SK and U6 snRNAs.

Product Categories/Family for MePCE recombinant protein

Recombinant Proteins; Transcription

NCBI and Uniprot Product Information

• NCBI GI #: 47271406
• NCBI GeneID: 56257
• NCBI Accession #: NP_062552.2
• NCBI GenBank Nucleotide #: NM_019606.5
• UniProt Accession #: Q7L2J0; Q9NPD4; B3KP86; D6W5V7
• Molecular Weight: 24,950 Da
• NCBI Official Full Name: 7SK snRNA methylphosphate capping enzyme isoform A
• NCBI Official Synonym Full Names: methylphosphate capping enzyme
• NCBI Official Symbol: MEPCE
• NCBI Official Synonym Symbols: BCDIN3
• NCBI Protein Information: 7SK snRNA methylphosphate capping enzyme; bin3 homolog; bin3, bicoid-interacting 3, homolog; bicoid-interacting protein 3 homolog
• UniProt Protein Name: 7SK snRNA methylphosphate capping enzyme
• Protein Family: 7SK snRNA methylphosphate capping enzyme
• UniProt Gene Name: MEPCE
• UniProt Synonym Gene Names: BCDIN3; MePCE; Bin3 homolog
• UniProt Entry Name: MEPCE_HUMAN

Uniprot Description

BCDIN3: a CDK9-associated enzyme that forms the distinctive gamma-methylphosphate cap structure of 7SK, a noncoding RNA that regulates CDK9 activity. An adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, leading to its stabilization. Component of the 7SK snRNP complex that contains P-TEFb (composed of CDK9 and CCNT1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Expressed in chronic myeloid leukemia cells, adrenal gland, brain, cerebellum, kidney, lung, mammary gland and testis. Weakly or not expressed in other tissues. Contains a Bin3 domain found in a number of protein methyltransferases that modify RNA-binding proteins.

Protein type: RNA processing; Transcription regulation; EC 2.1.1.-; EC 2.1.1.; Methyltransferase

Chromosomal Location of Human Ortholog: 7q22.1

Molecular Function: S-adenosylmethionine-dependent methyltransferase activity; RNA methyltransferase activity

Biological Process: snRNA metabolic process; snRNA modification; RNA methylation; negative regulation of transcription from RNA polymerase II promoter