Immunohistochemistry
Rabbit Tyrosine Hydroxylase Polyclonal Antibody | anti-TYH antibody
Rabbit anti Tyrosine Hydroxylase Polyclonal
Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml.
IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml.
Immunohistochemical analysis of FFPE Rat Hippocampus sections showing expression of Tyrosine Hydroxylase.
It was used as primary antibody, dilution 1:500, DAB staining.
Immunohistochemical analysis of FFPE mouse brain sections showing Substantia nigra neurons expressing Tyrosine Hydroxylase.
It was used as primary antibody, dilution 1:500, DAB staining.
Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Shelf Life: >12 months
Immunohistochemistry
Immunohistochemistry
• serine 129 phosphorylation reduces the ability of -synuclein to regulate Tyrosine Hydroxylase and protein phosphatase 2a in vitro and in vivo .J. Biol. Chem., Jun 2010; 285: 17648 - 17661.
• Tyrosine Hydroxylase is expressed during early heart development and is required for cardiac chamber formation .Cardiovasc Res, Jun 2010; 10.1093/cvr/cvq179.
• Tyrosine Hydroxylase deficiency: a treatable disorder of brain catecholamine biosynthesis .Brain, Jun 2010; 133: 1810 - 1822.
• electrophysiological and morphological characteristics and synaptic connectivity of Tyrosine Hydroxylase-expressing neurons in adult mouse striatum .J. Neurosci., May 2010; 30: 6999 - 7016.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described.
Protein type: EC 1.14.16.2; Endoplasmic reticulum; Mitochondrial; Vesicle; Amino Acid Metabolism - tyrosine; Oxidoreductase
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: synaptic vesicle; neuron projection; internal side of plasma membrane; smooth endoplasmic reticulum; mitochondrion; melanosome membrane; cytoplasm; dendrite; terminal button; perikaryon; cytoplasmic vesicle; cytosol; nucleus
Molecular Function: protein domain specific binding; amino acid binding; protein binding; enzyme binding; ferric iron binding; ferrous iron binding; dopamine binding; oxygen binding; tyrosine 3-monooxygenase activity
Biological Process: heart morphogenesis; heart development; response to lipopolysaccharide; dopamine biosynthetic process; phytoalexin metabolic process; norepinephrine biosynthetic process; dopamine biosynthetic process from tyrosine; catecholamine biosynthetic process; response to electrical stimulus; epinephrine biosynthetic process; response to pyrethroid; response to corticosterone stimulus; neurotransmitter biosynthetic process; response to light stimulus; anatomical structure morphogenesis; phthalate metabolic process; mating behavior; social behavior; eye photoreceptor cell development; organ morphogenesis; circadian sleep/wake cycle; response to ethanol; response to zinc ion; response to activity; cerebral cortex development; response to water deprivation; synaptic transmission, dopaminergic; response to peptide hormone stimulus; locomotory behavior; fatty acid metabolic process; response to estradiol stimulus; response to salt stress; regulation of heart contraction; sensory perception of sound; visual perception; glycoside metabolic process; response to nutrient levels; terpene metabolic process; sphingolipid metabolic process; eating behavior; response to amphetamine; multicellular organismal aging; isoquinoline alkaloid metabolic process; learning; response to herbicide; response to ether; synaptic vesicle amine transport; memory; pigmentation; response to hypoxia; embryonic camera-type eye morphogenesis
Disease: Segawa Syndrome, Autosomal Recessive
Similar Products
Product Notes
The TYH th (Catalog #AAA551076) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Tyrosine Hydroxylase Polyclonal reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Tyrosine Hydroxylase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA). ELISA: Use at a concentration range of 1-2ug/ml. Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml. IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml. Immunohistochemical analysis of FFPE Rat Hippocampus sections showing expression of Tyrosine Hydroxylase. It was used as primary antibody, dilution 1:500, DAB staining. Immunohistochemical analysis of FFPE mouse brain sections showing Substantia nigra neurons expressing Tyrosine Hydroxylase. It was used as primary antibody, dilution 1:500, DAB staining. Researchers should empirically determine the suitability of the TYH th for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tyrosine Hydroxylase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
