Testing Data #1
(ELISA analysis of recombinant chicken CD25 (supernatant from transfected COS cells, 1/3 dilution) with Mouse anti Chicken CD25 antibody (AV142). Supernatant from mock-transfected COS cells was used as negative control (Image courtesy of the Roslin Institute.))
Mouse CD25 Monoclonal Antibody | anti-CD25 antibody
MOUSE ANTI CHICKEN CD25
Applications
ELISA, Flow Cytometry, Functional Assay, Western Blot
Synonyms
CD25; Monoclonal Antibody; MOUSE ANTI CHICKEN CD25; anti-CD25 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
AV142
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
211
Applicable Applications for anti-CD25 antibody
ELISA (EIA), Flow cytometry (FC/FACS), Western Blot (WB)
Application Notes
ELISA: This product is suitable for use in direct ELISA applications.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Perservative Stabilisers
<0.1% Sodium Azide (NaN3)
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
Chicken CD25/hulg fusion protein
Buffer Solution
Phosphate buffered saline
Target Species
Chicken
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the Sp2/0 Ag14 Myeloma cell line.
Preparation and Storage
It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Testing Data #1
(ELISA analysis of recombinant chicken CD25 (supernatant from transfected COS cells, 1/3 dilution) with Mouse anti Chicken CD25 antibody (AV142). Supernatant from mock-transfected COS cells was used as negative control (Image courtesy of the Roslin Institute.))
Testing Data #1
(ELISA analysis of recombinant chicken CD25 (supernatant from transfected COS cells, 1/3 dilution) with Mouse anti Chicken CD25 antibody (AV142). Supernatant from mock-transfected COS cells was used as negative control (Image courtesy of the Roslin Institute.))
Testing Data #2
(Western blot analysis of chicken CD25/huIg fusion protein with Mouse anti Chicken CD25 (AV142). Recombinant huIg was used as negative control (Image courtesy of the Roslin Institute.))
Testing Data #2
(Western blot analysis of chicken CD25/huIg fusion protein with Mouse anti Chicken CD25 (AV142). Recombinant huIg was used as negative control (Image courtesy of the Roslin Institute.))
Testing Data #3
(Staining of ConA-stimulated chicken splenocytes with Mouse anti Chicken CD25 (AV142) conjugated with Alexa fluor-647(Image courtesy of the Roslin Institute.))
Testing Data #3
(Staining of ConA-stimulated chicken splenocytes with Mouse anti Chicken CD25 (AV142) conjugated with Alexa fluor-647(Image courtesy of the Roslin Institute.))
Related Product Information for anti-CD25 antibody
Mouse anti Chicken CD25 monoclonal antibody, clone AV142 specifically recognizes CD25, the chicken IL-2 Receptor alpha chain (IL-2Ralpha) which has been identified as the chicken homologue of human CD25 and is cited as ChCD25 in published literature. Chicken IL-2Ralpha is a cell surface antigen expressed by activated T-lymphocytes, monocytes/macrophages and thrombocytes. Studies have demonstrated that CD4+ve/CD25+ve and CD8+ve/CD25+ve cells are up-regulated following infection with avian influenza virus (H9N2) and may therefore have an important role in the immune response to avian influenza virus infection. Chicken IL-2Ralpha is the low affinity receptor for Interleukin-2. Clone AV142 is one of a range of antibodies that have been developed by the Roslin Institute as part of the Avian immulogy toolbox project. In addition to this anti chicken CD25 antibody, other antibodies to chicken CD antigens and cytokines from this project are now available from MyBioSource.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
interleukin 2 receptor, alpha
NCBI Official Synonym Full Names
interleukin 2 receptor, alpha
NCBI Official Symbol
IL2RA
NCBI Protein Information
interleukin 2 receptor, alpha; IL-2 alpha receptor; IL2 receptor alpha; interleukin-2 alpha receptor
Research Articles on CD25
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Product Notes
The CD25 (Catalog #AAA224689) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD25 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow cytometry (FC/FACS), Western Blot (WB). ELISA: This product is suitable for use in direct ELISA applications. Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Researchers should empirically determine the suitability of the CD25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD25, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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