Western Blot (WB)
(Western Blot: Sample: Human Serum.)
Rabbit anti-Human Cullin 1 Polyclonal Antibody | anti-CUL1 antibody
Cullin 1 (CUL1) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Human Serum.)
Western Blot (WB)
(Western Blot: Sample: Human Serum.)
Western Blot (WB)
(Western Blot: Sample: Recombinant CUL1, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant CUL1, Human.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded stomach tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded stomach tissue))
NCBI and Uniprot Product Information
Uniprot Description
CUL1: a core scaffolding component of multiple cullin-RING-based SCF (SKP1- CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. The cullin subunit serves as a rigid scaffold of SCF complexes. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex depends on the neddylation of the cullin subunit. Interacts with multiple F-box proteins in SCF complexes, directing the ubiquitination of many substrates. These F-box proteins and associated substrates include: BTRC and FBXW11 bind CTNNB1 and participate in Wnt signaling; FBXW11 bind phosphorylated NFKBIA; BTRC binds NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2; SKP2 binds phosphorylated p27kip and is involved in regulation of G1/S transition; SKP2 binds ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1; FBXW7 binds cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1; FBXW2 binds GCM1; FBXO32 binds MYOD1; FBXO7 binds BIRC2 and DLGAP5; FBXO33 binds YBX1; and Cyclin F binds CP110. Interacts with CHEK2, mediating its ubiquitination and regulating its function. Belongs to the cullin family.
Protein type: Cell cycle regulation; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 7q36.1
Cellular Component: cullin-RING ubiquitin ligase complex; cytosol; nucleoplasm; Parkin-FBXW7-Cul1 ubiquitin ligase complex; SCF ubiquitin ligase complex
Molecular Function: protein binding; ubiquitin protein ligase binding; ubiquitin-protein transferase activity
Biological Process: cell cycle arrest; cell proliferation; cellular iron ion homeostasis; Fc-epsilon receptor signaling pathway; G1/S transition of mitotic cell cycle; G2/M transition of mitotic cell cycle; negative regulation of cell proliferation; negative regulation of G2/M transition of mitotic cell cycle; NIK/NF-kappaB signaling; organ morphogenesis; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; post-translational protein modification; proteasomal ubiquitin-dependent protein catabolic process; protein monoubiquitination; protein polyubiquitination; protein ubiquitination; protein ubiquitination involved in ubiquitin-dependent protein catabolic process; SCF complex assembly; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; stimulatory C-type lectin receptor signaling pathway; stress-activated MAPK cascade; T cell receptor signaling pathway; viral process; Wnt signaling pathway
Research Articles on CUL1
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Product Notes
The CUL1 cul1 (Catalog #AAA2032881) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cullin 1 (CUL1) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cullin 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the CUL1 cul1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cullin 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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