Chromatin Immunoprecipitation (ChIP)
(Histone H3K4ac antibody (pAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of HeLa cell chromatin and 10 ug of antibody. ChIP DNA was sequenced on the Illumina NextSeq and 16 million sequence tags were mapped to identify Histone H3K4ac binding sites on chromosome 7.)
Rabbit Histone H3K4ac Polyclonal Antibody | anti-H3K4ac antibody
Histone H3K4ac antibody (pAb)
Reactivity
Human, Wide Range Predicted
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation, Dot Blot, Western Blot
Purity
Protein A Chromatography
Synonyms
Histone H3K4ac; Polyclonal Antibody; Histone H3K4ac antibody (pAb); anti-H3K4ac antibody
Host
Rabbit
Reactivity
Human, Wide Range Predicted
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-H3K4ac antibody
Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Seq (ChIP-Seq), Dot Blot (DB), Western Blot (WB)
Application Notes
Applications Validated:
ChIP-Seq: 10ug per ChIP
WB: 0.5-2ug/ml
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
ChIP-Seq: 10ug per ChIP
WB: 0.5-2ug/ml
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
Conjugation
Acetylated
Immunogen
This antibody was raised against a peptide containing acetyl lysine 4 of human Histone H3.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Chromatin Immunoprecipitation (ChIP)
(Histone H3K4ac antibody (pAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of HeLa cell chromatin and 10 ug of antibody. ChIP DNA was sequenced on the Illumina NextSeq and 16 million sequence tags were mapped to identify Histone H3K4ac binding sites on chromosome 7.)
Chromatin Immunoprecipitation (ChIP)
(Histone H3K4ac antibody (pAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of HeLa cell chromatin and 10 ug of antibody. ChIP DNA was sequenced on the Illumina NextSeq and 16 million sequence tags were mapped to identify Histone H3K4ac binding sites on chromosome 7.)
Western Blot (WB)
(Histone H3K4ac antibody (pAb) tested by Western blot. HeLa nuclear extract (20 ug) probed with Histone H3K4ac antibody (pAb) at 1 ug/ml. Lane 1: Acid extract of untreated HeLa cells. Lane 2: Acid extract of HeLa cells treated with sodium butyrate.)
Western Blot (WB)
(Histone H3K4ac antibody (pAb) tested by Western blot. HeLa nuclear extract (20 ug) probed with Histone H3K4ac antibody (pAb) at 1 ug/ml. Lane 1: Acid extract of untreated HeLa cells. Lane 2: Acid extract of HeLa cells treated with sodium butyrate.)
Dot Blot (DB)
(Histone H3K4ac antibody (pAb) tested by dot blot. Specificity Data: Dot blot analysis was used to confirm the specificity of H3K4ac antibody (pAb). Proteins and peptides were spotted onto nitrocellulose and probed with antibody at 1 ug/ml. The amount of protein/ peptide (in picomoles) spotted is indicated next to each row. Column 1: Rec. Histone H3 . Column 2: Rec. Histone H3K4ac . Column 4: Rec. Histone H3K18ac . Column 5: Histone H3K4ac peptide. Column 6: Histone H3K9ac peptide. Column 7: Histone H3K18ac.)
Dot Blot (DB)
(Histone H3K4ac antibody (pAb) tested by dot blot. Specificity Data: Dot blot analysis was used to confirm the specificity of H3K4ac antibody (pAb). Proteins and peptides were spotted onto nitrocellulose and probed with antibody at 1 ug/ml. The amount of protein/ peptide (in picomoles) spotted is indicated next to each row. Column 1: Rec. Histone H3 . Column 2: Rec. Histone H3K4ac . Column 4: Rec. Histone H3K18ac . Column 5: Histone H3K4ac peptide. Column 6: Histone H3K9ac peptide. Column 7: Histone H3K18ac.)
Related Product Information for anti-H3K4ac antibody
Short Description: Our Histone H3K4ac antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Dot blot and Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone acetylation is often associated with transcriptional activation. Lysine 4 of histone H3 can also be mono-, di- or trimethylated. This methylation is associated with transcriptional activation.
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone acetylation is often associated with transcriptional activation. Lysine 4 of histone H3 can also be mono-, di- or trimethylated. This methylation is associated with transcriptional activation.
Product Categories/Family for anti-H3K4ac antibody
NCBI and Uniprot Product Information
Molecular Weight
17kDa
Similar Products
Product Notes
The H3K4ac (Catalog #AAA389054) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H3K4ac antibody (pAb) reacts with Human, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3K4ac can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Seq (ChIP-Seq), Dot Blot (DB), Western Blot (WB). Applications Validated: ChIP-Seq: 10ug per ChIP WB: 0.5-2ug/ml Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. Researchers should empirically determine the suitability of the H3K4ac for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3K4ac, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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