Rabbit Chd7 Polyclonal Antibody | anti-CHD7 antibody

Chd7 Antibody

Cat. Num. AAA9612212

• Gene Names: CHD7; CRG; HH5; IS3; KAL5
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: Chd7; Polyclonal Antibody; Chd7 Antibody; ATP-dependent helicase CHD7; ATP-dependent helicase chromodomain helicase DNA binding protein 7; CHD-7; CHD7_HUMAN; Chromodomain helicase DNA binding protein 7; chromodomain helicase DNA binding protein 7 isoform CRA_e; Chromodomain-helicase-DNA-binding protein 7; FLJ20357; FLJ20361; HH5; IS3; KAL5; KIAA1416; anti-CHD7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Chd7 Antibody detects endogenous levels of total Chd7.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 2997

• Applicable Applications for anti-CHD7 antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human Chd7, corresponding to a region within C-terminal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from SK-N-SH, using Chd7 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-CHD7 antibody

Function: Probable transcription regulator. Maybe involved in the in 45S precursor rRNA production.

Subcellular Location: Nucleus. Nucleus>Nucleolus.

Tissue Specificity: Widely expressed in fetal and adult tissues.

Subunit Structure: May interact with CTCF (PubMed:17603073). Interacts with CHD8 (PubMed:20453063). Interacts with FAM124B (PubMed:23285124). Found in a complex composed of AGO2, CHD7 and FAM172A (By similarity).

Similarity: Belongs to the SNF2/RAD54 helicase family.

NCBI and Uniprot Product Information

• NCBI GI #: 54112403
• NCBI GeneID: 55636
• NCBI Accession #: NP_060250.2
• NCBI GenBank Nucleotide #: NP_060250.2
• UniProt Accession #: Q9P2D1
• Molecular Weight: Observed Molecular Weight: (Observed)335kD.; Predicted Molecular Weight: (Calculated)336kDa.
• NCBI Official Full Name: chromodomain-helicase-DNA-binding protein 7 isoform 1
• NCBI Official Synonym Full Names: chromodomain helicase DNA binding protein 7
• NCBI Official Symbol: CHD7
• NCBI Official Synonym Symbols: CRG; HH5; IS3; KAL5
• NCBI Protein Information: chromodomain-helicase-DNA-binding protein 7
• UniProt Protein Name: Chromodomain-helicase-DNA-binding protein 7
• Protein Family: Chromodomain-helicase-DNA-binding protein
• UniProt Gene Name: CHD7
• UniProt Synonym Gene Names: KIAA1416; CHD-7
• UniProt Entry Name: CHD7_HUMAN

NCBI Description

This gene encodes a protein that contains several helicase family domains. Mutations in this gene have been found in some patients with the CHARGE syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]

Uniprot Description

CHD-7: Probable transcription regulator. May interact with CTCF. Interacts with CHD8. Widely expressed in fetal and adult tissues. Belongs to the SNF2/RAD54 helicase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; DNA-binding; EC 3.6.4.12; Helicase

Chromosomal Location of Human Ortholog: 8q12.2

Cellular Component: nucleoplasm; nucleolus; nucleus

Molecular Function: protein binding; DNA binding; chromatin binding; helicase activity; ATP binding

Biological Process: limb development; heart morphogenesis; central nervous system development; olfactory behavior; blood circulation; positive regulation of multicellular organism growth; palate development; female genitalia development; regulation of growth hormone secretion; olfactory nerve development; embryonic hindlimb morphogenesis; adult walking behavior; sensory perception of sound; regulation of neurogenesis; regulation of transcription, DNA-dependent; skeletal development; T cell differentiation; adult heart development; inner ear morphogenesis; transcription, DNA-dependent; in utero embryonic development; olfactory bulb development; semicircular canal morphogenesis; cranial nerve development; chromatin modification; genitalia development; retina development in camera-type eye; artery morphogenesis; cognition; nose development; rRNA processing

Disease: Charge Syndrome; Tracheoesophageal Fistula With Or Without Esophageal Atresia; Hypogonadotropic Hypogonadism 5 With Or Without Anosmia