Western Blot (WB)
(Histone H3 monomethyl Arg8 pAb tested by Western blot. HeLa cell nuclear extract (20 ug) probed with Histone H3 monomethyl Arg8 pAb (1:500 dilution).)
Rabbit Histone H3R8me1 Polyclonal Antibody | anti-H3R8me1 antibody
Histone H3R8me1 antibody (pAb)
Reactivity
Human, Wide Range Predicted
Applications
Dot Blot, Western Blot
Synonyms
Histone H3R8me1; Polyclonal Antibody; Histone H3R8me1 antibody (pAb); anti-H3R8me1 antibody
Host
Rabbit
Reactivity
Human, Wide Range Predicted
Clonality
Polyclonal
Form/Format
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-H3R8me1 antibody
Dot Blot (DB), Western Blot (WB)
Application Notes
Applications Validated:
WB: 1:500-1:2,000 dilution
WB: 1:500-1:2,000 dilution
Conjugation
Methylated
Immunogen
This Histone H3 monomethyl Arg8 antibody was raised against a peptide containing monomethyl-Arg8 of human histone H3.
Abs Residue
Arg
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(Histone H3 monomethyl Arg8 pAb tested by Western blot. HeLa cell nuclear extract (20 ug) probed with Histone H3 monomethyl Arg8 pAb (1:500 dilution).)
Western Blot (WB)
(Histone H3 monomethyl Arg8 pAb tested by Western blot. HeLa cell nuclear extract (20 ug) probed with Histone H3 monomethyl Arg8 pAb (1:500 dilution).)
Dot Blot (DB)
(Histone H3 monomethyl Arg8 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 monomethyl Arg8 pAb for monomethyl-arginine 8 of histone H3. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H3 monomethyl Arg8 pAb at 1:1,000. The amount of peptide (picomoles) spotted is indicated next to each row. Top Panel: Lane 1: unmodified Arg2 peptide. Lane 2: monomethyl-Arg2 H3 peptide. Lane 3: dimethyl-Arg2 H3 (symmetric) peptide. Lane 4: dimethyl-Arg2 H3 (asymmetric) peptide. Lane 5: unmodified Arg8 peptide. Lane 6: monomethyl-Arg8 H3 peptide. Lane 7: dimethyl-Arg8 H3 (symmetric) peptide. Lane 8: dimethyl-Arg8 H3 (asymmetric) peptide. Bottom Panel: Lane 1: unmodified Arg17 H3 peptide. Lane 2: monomethyl-Arg17 peptide. Lane 3: dimethyl-Arg17 H3 (symmetric) peptide. Lane 4: dimethyl-Arg17 H3 (asymmetric) peptide. Lane 5: unmodified Arg26 peptide. Lane 6: monomethyl-Arg26 H3 peptide. Lane 7: dimethyl-Arg26 H3 (symmetric) peptide. Lane 8: dimethyl-Arg26 H3 (asymmetric) peptide.)
Dot Blot (DB)
(Histone H3 monomethyl Arg8 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 monomethyl Arg8 pAb for monomethyl-arginine 8 of histone H3. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H3 monomethyl Arg8 pAb at 1:1,000. The amount of peptide (picomoles) spotted is indicated next to each row. Top Panel: Lane 1: unmodified Arg2 peptide. Lane 2: monomethyl-Arg2 H3 peptide. Lane 3: dimethyl-Arg2 H3 (symmetric) peptide. Lane 4: dimethyl-Arg2 H3 (asymmetric) peptide. Lane 5: unmodified Arg8 peptide. Lane 6: monomethyl-Arg8 H3 peptide. Lane 7: dimethyl-Arg8 H3 (symmetric) peptide. Lane 8: dimethyl-Arg8 H3 (asymmetric) peptide. Bottom Panel: Lane 1: unmodified Arg17 H3 peptide. Lane 2: monomethyl-Arg17 peptide. Lane 3: dimethyl-Arg17 H3 (symmetric) peptide. Lane 4: dimethyl-Arg17 H3 (asymmetric) peptide. Lane 5: unmodified Arg26 peptide. Lane 6: monomethyl-Arg26 H3 peptide. Lane 7: dimethyl-Arg26 H3 (symmetric) peptide. Lane 8: dimethyl-Arg26 H3 (asymmetric) peptide.)
Related Product Information for anti-H3R8me1 antibody
Short Description: Our Histone H3R8me1 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot and Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
Background: Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone H3 Arg8, methylated by PRMT enzymes, is involved in nuclear-receptor-mediated transcriptional activation.
Background: Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone H3 Arg8, methylated by PRMT enzymes, is involved in nuclear-receptor-mediated transcriptional activation.
Product Categories/Family for anti-H3R8me1 antibody
NCBI and Uniprot Product Information
Molecular Weight
17kDa
Similar Products
Product Notes
The H3R8me1 (Catalog #AAA388536) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H3R8me1 antibody (pAb) reacts with Human, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3R8me1 can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), Western Blot (WB). Applications Validated: WB: 1:500-1:2,000 dilution. Researchers should empirically determine the suitability of the H3R8me1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3R8me1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
