Shipping Temp: Dry Ice
SDS-Page
(Recombinant SUV420H2 (2-281) protein 10% SDS-PAGE Coomassie staining MW: 58.1 kDa Purity: > 90%)
Western Blot (WB)
(Recombinant SUV420H2 (2-281) activity assay Recombinant Nucleosomes (H3.1) were incubated with SUV420H2 (2-281) protein. Reaction products were run on SDS-PAGE, and detected by Western Blot using Anti-H4K20me3.Results show the activity of SUV420H2 (2-281) with nucleosomes as substrate is better than with histone octamers.)
Background: SUV420H2 (Suppressor Of Variegation 4-20 Homolog 2, also known as KMT5C) is a histone methyltransferase that specifically trimethylates nucleosomal histone H4 on lysine 20 (H4K20). H4K20me3 is a histone marker that represents epigenetic transcriptional repression. SUV420H2 mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. SUV420H2 is targeted to histone H3 via its interaction with RB1 family proteins.
NCBI and Uniprot Product Information
Uniprot Description
SUV420H2: Histone methyltransferase that specifically trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. SUV420H1 is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2). Belongs to the histone-lysine methyltransferase family. Suvar4-20 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Methyltransferase; EC 2.1.1.43; Amino Acid Metabolism - lysine degradation; Methyltransferase, protein lysine
Chromosomal Location of Human Ortholog: 19q13.42
Cellular Component: nucleoplasm; centric heterochromatin; nuclear heterochromatin; condensed nuclear chromosome, pericentric region
Molecular Function: protein binding; histone lysine N-methyltransferase activity (H4-K20 specific)
Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent
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Product Notes
The SUV420H2 suv420h2 (Catalog #AAA389076) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SUV420H2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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