Mouse anti-Rat, Yeast Glucose Regulated Protein 78 Monoclonal Antibody

Glucose Regulated Protein 78 (Grp78, BiP, KDEL)

Cat. Num. AAA600646

• Reactivity: Rat, Yeast
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Glucose Regulated Protein 78; Monoclonal Antibody; Glucose Regulated Protein 78 (Grp78; BiP; KDEL); Anti -Glucose Regulated Protein 78 (Grp78; anti-Glucose Regulated Protein 78 antibody

• Host: Mouse
• Reactivity: Rat, Yeast
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 4E87
• Specificity: Recognizes rat Glucose Regulated Protein 78. Reacts with proteins containing the KDEL sequence. Detects three bands of ~94kD, ~78kD and ~55kD by Western Blot.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.

• Applicable Applications for anti-Glucose Regulated Protein 78 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Dilution: Western Blot (Colormetric): 1:1000; Immunohistochemistry: Paraffin sections; Optimal dilutions to be determined by researcher.
• Immunogen: Synthetic peptide corresponding to aa649-654, S649SEKDEL654, from rat Glucose Regulated Protein 78
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis using MBS600646: Lane 1: Grp78 (BiP) recombinant protein, Lane 2: RK-13, Lane 3: Mouse liver microsomes, Lane 4: Rat liver microsomes, Lane 5: HeLa Cell Lysate (heat shocked))

Immunofluorescence (IF)

(Immunofluorescence analysis of endoplasmic reticulum staining of mouse C2C12 myoblasts transfected with wild type mouse ADAM12 using MBS600646)

Immunohistochemistry (IHC)

(Immunohistochemical staining of human spleen tissue using MBS600646.)

Related Product Information for anti-Glucose Regulated Protein 78 antibody

The tetrapeptide, KDEL located at the carboxy-terminal sequences of luminal proteins is a retrieval motif essential for the precise sorting of these proteins along the secretory pathway. The KDEL proteins perform essential functions in the ER related to protein folding as well as assembly. The localization of chaperones and other soluble proteins to the endoplasmic reticulum (ER) is achieved by their continuous retrieval from post-ER compartments by the KDEL receptor (Erd2p) which is a membrane protein localized in the Golgi apparatus. The KDEL receptor has extensive sequence identity to the yeast Erd2p receptor for proteins bearing the tetrapeptide, HDEL, the yeast ER retention signal. Earlier studies using yeast mutants with deleted HDEL receptors had shown that this receptor was essential for retrieval of HDEL proteins and possibly for regulating transport through the Golgi complex. The regulation of transport through the KDEL receptor appears to be similar to many signal transduction processes in which membrane receptors act through a GTPase-activating protein (GAP that promotes hydrolysis of GTP to GDP) or a quanine nucleotide exchange factor (GEP that enhances exchange of GDP for GTP) of key small GTPases to regulate dfferent cellular functions. Recent data demonstrates that the non-catalytic domain of GAP that is essential for GAP activity in vivo, mediates the recruitment of cytosolic GAP to membranes and its interaction with the KDEL receptor. This interaction with the KDEL receptor is critical for GAP to act on ADP-ribosylation factor 1 (ARF1) which is a key regulator of transport in the secretory system.

Product Categories/Family for anti-Glucose Regulated Protein 78 antibody

Antibodies; Abs to Heat Shock Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 170386
• NCBI Official Full Name: glucose-regulated protein 78