Western Blot (WB)
(INHAT-1/TAF-1a/TAF-1b pAb tested by Western blot. INHAT-1 detection by Western blot. The analysis was performed using Jurkat whole-cell extract and INHAT-1/TAF-1a/TAF-1b pAb at a 2 ug/ml dilution.)
Rabbit INHAT-1/TAF-1a/TAF-1b Polyclonal Antibody | anti-INHAT-1/TAF-1a/TAF-1b antibody
INHAT-1/TAF-1a/TAF-1b antibody (pAb)
Gene Names
TAF1A; SL1; RAFI48; TAFI48; MGC:17061
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Western Blot
Purity
Affinity Purified
Synonyms
INHAT-1/TAF-1a/TAF-1b; Polyclonal Antibody; INHAT-1/TAF-1a/TAF-1b antibody (pAb); TAF1A; SL1; RAFI48; TAFI48; anti-INHAT-1/TAF-1a/TAF-1b antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
PBS containing 0.02% sodium azide and 0.1mg/ml BSA. Sodium azide is highly toxic.
Concentration
0.5ug/ul (varies by lot)
Applicable Applications for anti-INHAT-1/TAF-1a/TAF-1b antibody
Immunocytochemistry (ICC), Western Blot (WB)
Application Notes
Applications Validated:
WB: 1-2ug/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
WB: 1-2ug/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
Immunogen
This INHAT-1/TAF-1a/TAF-1b antibody was raised against a mixture of synthetic peptides corresponding to amino acid residues 66-81 and 135-151 of human INHAT-1/TAF-1a/TAF-1b.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(INHAT-1/TAF-1a/TAF-1b pAb tested by Western blot. INHAT-1 detection by Western blot. The analysis was performed using Jurkat whole-cell extract and INHAT-1/TAF-1a/TAF-1b pAb at a 2 ug/ml dilution.)
Western Blot (WB)
(INHAT-1/TAF-1a/TAF-1b pAb tested by Western blot. INHAT-1 detection by Western blot. The analysis was performed using Jurkat whole-cell extract and INHAT-1/TAF-1a/TAF-1b pAb at a 2 ug/ml dilution.)
Related Product Information for anti-INHAT-1/TAF-1a/TAF-1b antibody
Short Description: Our INHAT-1/TAF-1a/TAF-1b antibody (pAb) was raised in a Rabbit host. It has been validated for use in Immunocytochemistry and Western blot; it has been shown to react with Human, Mouse and Rat samples.
Background: INHAT (also designated TAF-1a, TAF-1b and Inhibitor of Histone Acetyltransferases complex) binds to histones and masks them from being substrates for histone acetyltransferases (HATs). Endogenous INHAT subunits, which include the Set/TAF-Ibeta oncoprotein, and pp32 associate with chromatin in vivo and can block coactivator-mediated transcription when transfected in cells. Single phosphorylations within the Histone H3 tail blocks binding of INHAT, as well as simultaneous acetylation of multiple lysine residues.
Background: INHAT (also designated TAF-1a, TAF-1b and Inhibitor of Histone Acetyltransferases complex) binds to histones and masks them from being substrates for histone acetyltransferases (HATs). Endogenous INHAT subunits, which include the Set/TAF-Ibeta oncoprotein, and pp32 associate with chromatin in vivo and can block coactivator-mediated transcription when transfected in cells. Single phosphorylations within the Histone H3 tail blocks binding of INHAT, as well as simultaneous acetylation of multiple lysine residues.
Product Categories/Family for anti-INHAT-1/TAF-1a/TAF-1b antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,615 Da
NCBI Official Full Name
TATA box-binding protein-associated factor RNA polymerase I subunit A isoform 1
NCBI Official Synonym Full Names
TATA box binding protein (TBP)-associated factor, RNA polymerase I, A, 48kDa
NCBI Official Symbol
TAF1A
NCBI Official Synonym Symbols
SL1; RAFI48; TAFI48; MGC:17061
NCBI Protein Information
TATA box-binding protein-associated factor RNA polymerase I subunit A; RNA polymerase I-specific TBP-associated factor 48 kDa; SL1, 48kD subunit; TATA box binding protein (TBP)-associated factor, RNA polymerase I, A, 48kD; TATA box-binding protein-associa
UniProt Protein Name
TATA box-binding protein-associated factor RNA polymerase I subunit A
UniProt Gene Name
TAF1A
UniProt Synonym Gene Names
TAFI48; TBP-associated factor 1A
UniProt Entry Name
TAF1A_HUMAN
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Product Notes
The INHAT-1/TAF-1a/TAF-1b taf1a (Catalog #AAA388402) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The INHAT-1/TAF-1a/TAF-1b antibody (pAb) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's INHAT-1/TAF-1a/TAF-1b can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Western Blot (WB). Applications Validated: WB: 1-2ug/ml dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Researchers should empirically determine the suitability of the INHAT-1/TAF-1a/TAF-1b taf1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "INHAT-1/TAF-1a/TAF-1b, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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