Western Blot (WB)
(INHAT-2/pp32 pAb tested by Western blot. Detection of INHAT-2 by Western blot. The analysis was performed using HeLa whole-cell extract and INHAT-2/pp32 pAb at a 1:1,000 dilution.)
Rabbit anti-Human INHAT-2/pp32 Polyclonal Antibody | anti-INHAT-2/pp32 antibody
INHAT-2/pp32 antibody (pAb)
Gene Names
ANP32A; LANP; MAPM; PP32; HPPCn; PHAP1; PHAPI; I1PP2A; C15orf1
Reactivity
Human
Applications
Western Blot
Purity
Affinity Purified
Synonyms
INHAT-2/pp32; Polyclonal Antibody; INHAT-2/pp32 antibody (pAb); ANP32A; LANP; MAPM; PP32; HPPCn; PHAP1; PHAPI; I1PP2A; C15orf1; anti-INHAT-2/pp32 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
PBS containing 0.02% sodium azide and 0.1mg/ml BSA. Sodium azide is highly toxic.
Applicable Applications for anti-INHAT-2/pp32 antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 1:500-1:1,000 dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
WB: 1:500-1:1,000 dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
Immunogen
This INHAT-2/pp32 antibody was raised against a mixture of synthetic peptides corresponding to amino acid residues 87-103 and 231-246 of human INHAT-2/pp32.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(INHAT-2/pp32 pAb tested by Western blot. Detection of INHAT-2 by Western blot. The analysis was performed using HeLa whole-cell extract and INHAT-2/pp32 pAb at a 1:1,000 dilution.)
Western Blot (WB)
(INHAT-2/pp32 pAb tested by Western blot. Detection of INHAT-2 by Western blot. The analysis was performed using HeLa whole-cell extract and INHAT-2/pp32 pAb at a 1:1,000 dilution.)
Related Product Information for anti-INHAT-2/pp32 antibody
Short Description: Our INHAT-2/pp32 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot; it has been shown to react with Human samples.
Background: INHAT (pp32) binds to histones and masks them from being substrates for histone acetyltransferases (HATs). Endogenous INHAT subunits, which include the Set/TAF-I beta oncoprotein, and pp32 associate with chromatin in vivo and can block coactivator mediated transcription when transfected in cells. Single phosphorylations within the Histone H3 tail blocks binding of INHAT, as well as simultaneous acetylation of multiple lysine residues.
Background: INHAT (pp32) binds to histones and masks them from being substrates for histone acetyltransferases (HATs). Endogenous INHAT subunits, which include the Set/TAF-I beta oncoprotein, and pp32 associate with chromatin in vivo and can block coactivator mediated transcription when transfected in cells. Single phosphorylations within the Histone H3 tail blocks binding of INHAT, as well as simultaneous acetylation of multiple lysine residues.
Product Categories/Family for anti-INHAT-2/pp32 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30.7kDa (269aa), confirmed by MALDI-TOF
NCBI Official Full Name
acidic leucine-rich nuclear phosphoprotein 32 family member A
NCBI Official Synonym Full Names
acidic nuclear phosphoprotein 32 family member A
NCBI Official Symbol
ANP32A
NCBI Official Synonym Symbols
LANP; MAPM; PP32; HPPCn; PHAP1; PHAPI; I1PP2A; C15orf1
NCBI Protein Information
acidic leucine-rich nuclear phosphoprotein 32 family member A
UniProt Protein Name
Acidic leucine-rich nuclear phosphoprotein 32 family member A
UniProt Gene Name
ANP32A
UniProt Synonym Gene Names
C15orf1; LANP; MAPM; PHAP1; pp32; LANP; PHAPI
UniProt Entry Name
AN32A_HUMAN
Uniprot Description
ANP32A: Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase- independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1- mediated transcriptional repression. Belongs to the ANP32 family.
Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 15q23
Cellular Component: nucleoplasm; endoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm; nucleus
Molecular Function: protein binding
Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; gene expression; nucleocytoplasmic transport
Research Articles on INHAT-2/pp32
Similar Products
Product Notes
The INHAT-2/pp32 anp32a (Catalog #AAA388409) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The INHAT-2/pp32 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's INHAT-2/pp32 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1:500-1:1,000 dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Researchers should empirically determine the suitability of the INHAT-2/pp32 anp32a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "INHAT-2/pp32, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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