Testing Data
Mouse Poly(ADP-Ribose) Polymer Monoclonal Antibody
Anti-Poly(ADP-Ribose) Polymer, Clone 10H
Reactivity
Does not crossreact with ADP-ribose, 5'-AMP, or yeast RNA as tested by ELISA.
Applications
Immunocytochemistry, Western Blot, Immunoprecipitation, ELISA
Synonyms
Poly(ADP-Ribose) Polymer; Monoclonal Antibody; Anti-Poly(ADP-Ribose) Polymer; Clone 10H; Mouse Monoclonal Antibody; anti-Poly(ADP-Ribose) Polymer antibody
Host
Mouse
Reactivity
Does not crossreact with ADP-ribose, 5'-AMP, or yeast RNA as tested by ELISA.
Clonality
Monoclonal
Isotype
IgG3kappa
Clone Number
10H.
Form/Format
1 mg/ml of IgG purified from mouse ascites by protein A chromatography. Prepared in 20 mM Tris, pH 7.4, 150 mM NaCl, and 0.02% sodium azide. Carrier-protein free.
Applicable Applications for anti-Poly(ADP-Ribose) Polymer antibody
Immunocytochemistry (ICC), Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA)
Application Notes
Immunocytochemistry 5-20 ug/ml Western Blot (10 ug/ml using colorimetric methods; <2 ug/ml for ECL). Immunoprecipitation, ELISA.
Hybridoma
Balb/c x NS-1.
Immunogen
Poly(ADP-ribose) mixed with methylated bovine serum albumin.
Preparation and Storage
Stable for 1 year from date of shipment when stored at -20 or -70 degree C. Stable for 1 month at 4 degree C. Avoid freeze/thaw cycles.
Testing Data
Testing Data
Related Product Information for anti-Poly(ADP-Ribose) Polymer antibody
Background: Poly(ADP-ribose) or "pADPr" is a polymer synthesized by a class of enzymes named poly(ADP-ribose) polymerase's (PARP). Using NAD+ as substrate, PARP catalyzes the formation of the polymer pADPr, with chain lengths ranging from 2 to 300 residues, containing approximately 2% branching in the chain. pADPr becomes attached to nuclear proteins, and to PARP itself (automodification). Under normal conditions, cells display low basal level of pADPr polymer, which can dramatically increase in cells exposed to DNA damaging agents (irradiation, alkylation, etc.). This increase of polymer synthesis is usually transient and is followed by a rapid degradation phase with a short half life which can be less than 1 min. The low endogenous level of polymer in unstimulated cells and its rapid catabolism during DNA damage has been ascribed to high activity of the polymer catabolizing enzyme poly(ADP-ribose) glycohydrolyase (PARG).
References
H. Kawamitsu et al. (1984) Biochemistry 23, 3771
G.M. Shah et al. (1995) Anal. Biochem. 227, 1 D. D'Amours et al. (1997) Anal. Biochem. 249, 106 E.B. Affar et al. (1998) Anal. Biochem. 259, 280 S. Smith et al. (1998) Science 282, 1484 D.M. Simbulan-Rosenthal et al. (1998) J. Biol. Chem. 273, 13703 D. D'Amours et al. (1999) Biochem. J. 342, 249 F. Sallmann et al. (2000) J. Biol. Chem. 275, 15504 L. Virag and C. Szabo (2002) Pharmacol. Rev. 54, 375
G.M. Shah et al. (1995) Anal. Biochem. 227, 1 D. D'Amours et al. (1997) Anal. Biochem. 249, 106 E.B. Affar et al. (1998) Anal. Biochem. 259, 280 S. Smith et al. (1998) Science 282, 1484 D.M. Simbulan-Rosenthal et al. (1998) J. Biol. Chem. 273, 13703 D. D'Amours et al. (1999) Biochem. J. 342, 249 F. Sallmann et al. (2000) J. Biol. Chem. 275, 15504 L. Virag and C. Szabo (2002) Pharmacol. Rev. 54, 375
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Product Notes
The Poly(ADP-Ribose) Polymer (Catalog #AAA376003) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Poly(ADP-Ribose) Polymer, Clone 10H reacts with Does not crossreact with ADP-ribose, 5'-AMP, or yeast RNA as tested by ELISA. and may cross-react with other species as described in the data sheet. AAA Biotech's Poly(ADP-Ribose) Polymer can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA). Immunocytochemistry 5-20 ug/ml Western Blot (10 ug/ml using colorimetric methods; <2 ug/ml for ECL). Immunoprecipitation, ELISA. Researchers should empirically determine the suitability of the Poly(ADP-Ribose) Polymer for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Poly(ADP-Ribose) Polymer, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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