Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Poly ADP ribose polymerase (PARP) Standard Curve.)
Human Poly ADP Ribose Polymerase (PARP) ELISA Kit | PARP elisa kit
Human Poly ADP Ribose Polymerase (PARP) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Poly ADP ribose polymerase (PARP) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Poly ADP ribose polymerase (PARP) Standard Curve.)
Background: PARP is a protein involved in a number of cellular processes involving mainly DNA repair and programmed cell death.RP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like caspases in vitroand is one of the main cleavage targets of caspase-3 in vivo. In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa). PARP1 is a chromatin-associated enzyme, poly-ADP ribose polymerase-1, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. PARP1 has a role in repair of single-stranded DNA (ssDNA) breaks.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]
Uniprot Description
PARP1: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP- ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and ZNF423. Interacts (when poly-ADP- ribosylated) with CHD1L. Interacts with the DNA polymerase alpha catalytic subunit POLA1; this interaction functions as part of the control of replication fork progression. Interacts with EEF1A1, RNF4 and TXK.
Protein type: Nucleolus; Nuclear envelope; DNA repair, damage; Nuclear receptor co-regulator; EC 2.4.2.30; Transferase
Chromosomal Location of Human Ortholog: 1q41-q42
Cellular Component: nucleoplasm; transcription factor complex; membrane; nucleolus; nuclear envelope; nucleus
Molecular Function: identical protein binding; protein binding; enzyme binding; DNA binding; zinc ion binding; protein N-terminus binding; NAD binding; transcription factor binding; protein kinase binding; NAD+ ADP-ribosyltransferase activity
Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; macrophage differentiation; transcription, DNA-dependent; DNA damage response, detection of DNA damage; negative regulation of transcription from RNA polymerase II promoter; DNA repair; protein autoprocessing; protein amino acid ADP-ribosylation; cellular response to insulin stimulus; base-excision repair; transforming growth factor beta receptor signaling pathway; double-strand break repair; positive regulation of transcription from RNA polymerase II promoter; gene expression; regulation of growth rate; telomere maintenance
Research Articles on PARP
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Product Notes
The Human PARP parp1 (Catalog #AAA9714967) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714967 ELISA Kit recognizes Human PARP. It is sometimes possible for the material contained within the vial of "Poly ADP Ribose Polymerase (PARP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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