Poly-ADP-ribose Protein

Poly-ADP-ribose Affinity Resin

Cat. Num. AAA376018

• Applications: Western Blot
• Purity: GST-macrodomain fusion protein purity >95% by SDS-PAGE.
• Synonyms: Poly-ADP-ribose; Poly-ADP-ribose Affinity Resin; Poly-ADP-ribose protein

• Purity/Purification: GST-macrodomain fusion protein purity >95% by SDS-PAGE.
• Form/Format: Each vial contains 1mg purified GST-macrodomain- fusion protein bound to approximately 100uL packed volume of glutathione beads in 1 mL buffer containing phosphate buffered saline with 1 mM EDTA, 1% Triton X-100, and 0.02% sodium azide.

• Applicable Applications for Poly-ADP-ribose protein: Western Blot (WB)
• Application Notes: Use 20uL (20ug) suspended resin to affinity purify/pull-down poly-ADP-ribose modified proteins in 0.15-1mg cell and tissue extracts. Analyze by Western blotting using protein specific antibodies to probe the immunoblot. Each 1mL vial is sufficient for analysis of ~50 samples.
• Preparation and Storage: Stable for 6 months from date of shipment when stored at 4 degree C. DO NOT FREEZE!

Testing Data

Testing Data #2

Related Product Information for Poly-ADP-ribose protein

Background: Poly-ADP-ribose (PAR) Affinity Resin Set is designed for the isolation and study of intracellular poly-ADP-ribosylated (PARylated) proteins. Through the use of a highly specific PAR affinity resin, PARylated proteins are isolated from cell or tissue lysates without the use of anti-PAR antibodies. The resin bound proteins can be eluted from the affinity resin, and analyzed by immunoblotting or other methods. Af1521 is a thermopohilic protein from Archaeoglobus fulgidus, and contains a conserved ~190 amino acid domain known as the macro domain. Macrodomains are found in a wide variety of organisms including bacteria, viruses, and vertebrates. Expressed and purified macrodomains from Af1521, Alc1, macroH2A and Bal/PARP9 proteins have been shown to bind polymeric ADP-ribose modified proteins with high specificity and affinity.
Product Description: The Poly-ADP-ribose Binding Domain Resin Set contains 1 each of the following: Poly-ADP-ribose Binding Domain Resin 1mL (1mg fusion protein supplied as a slurry containing ~100uL packed resin). Poly-ADP-ribose Neg Control Resin 1mL (1mg fusion protein supplied as a slurry containing ~100uL packed resin).PAR Affinity Resin is highly purified GST-Af1521 macrodomain fusion protein contruct expressed in E. coli, and bound to glutathione beads. It is useful for affinity purification (pulldown) of PARsylated proteins as well as PAR polymer. The Af1521 macrodomain protein is also reported to bind mono-ADP-ribosylated proteins and ADP-ribose. PAR Negative Control Resin had two gly to asp substitutions, which abolish PAR binding. The negative control resin is useful to control for non-specific binding, and its use is optional.

References

J-P. Gagne et al. (2012) Nucleic Acids Res 40: 7788