Rabbit anti-Human FCN3 Polyclonal Antibody | anti-FCN3 antibody

FCN3, CT (FCN3, FCNH, HAKA1, Ficolin-3, Collagen/fibrinogen domain-containing lectin 3 p35, Collagen/fibrinogen domain-containing protein 3, Hakata antigen) (FITC)

Cat. Num. AAA6298706

• Gene Names: FCN3; FCNH; HAKA1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FCN3; Polyclonal Antibody; CT (FCN3; FCNH; HAKA1; Ficolin-3; Collagen/fibrinogen domain-containing lectin 3 p35; Collagen/fibrinogen domain-containing protein 3; Hakata antigen) (FITC); Hakata antigen; anti-FCN3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-FCN3 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: FCN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 213-243 amino acids from the C-terminal region of human FCN3.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FCN3 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the FCN3 antibody detected the FCN3 protein (arrow).)

Immunohistochemistry (IHC)

(FCN3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FCN3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(FCN3 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-FCN3 antibody

Ficolins are a group of proteins which consist of a collagen-like domain and a fibrinogen-like domain. In human serum, there are two types of ficolins, both of which have lectin activity. The protein encoded by this gene is a thermolabile beta-2-macroglycoprotein found in all human serum and is a member of the ficolin/opsonin p35 lectin family. The protein, which was initially identified based on its reactivity with sera from patients with systemic lupus erythematosus, has been shown to have a calcium-independent lectin activity. The protein can activate the complement pathway in association with MASPs and sMAP, thereby aiding in host defense through the activation of the lectin pathway. Alternative splicing occurs at this locus and two variants, each encoding a distinct isoform, have been identified.

Product Categories/Family for anti-FCN3 antibody

Antibodies; Fibrinogen

References

Davila, S., et al. Genes Immun. 11(3):232-238(2010). Andersen, T., et al. J. Rheumatol. 36(4):757-759(2009). Ruskamp, J.M., et al. Clin. Exp. Immunol. 155(3):433-440(2009). Lacroix, M., et al. J. Immunol. 182(1):456-465(2009). Munthe-Fog, L., et al. Mol. Immunol. 45(9):2660-2666(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 27754778
• NCBI GeneID: 8547
• NCBI Accession #: NP_775628.1; NP_003656.2
• NCBI GenBank Nucleotide #: NM_173452.1
• UniProt Accession #: O75636; Q6IBJ5; Q8WW86
• Molecular Weight: 31,678 Da
• NCBI Official Full Name: ficolin-3 isoform 2
• NCBI Official Synonym Full Names: ficolin (collagen/fibrinogen domain containing) 3
• NCBI Official Symbol: FCN3
• NCBI Official Synonym Symbols: FCNH; HAKA1
• NCBI Protein Information: ficolin-3; H-ficolin; ficolin 3; collagen/fibrinogen domain-containing protein 3; collagen/fibrinogen domain-containing lectin 3 p35; ficolin (collagen/fibrinogen domain containing) 3 (Hakata antigen)
• UniProt Protein Name: Ficolin-3
• Protein Family: Ficolin
• UniProt Gene Name: FCN3
• UniProt Synonym Gene Names: FCNH; HAKA1
• UniProt Entry Name: FCN3_HUMAN

Uniprot Description

FCN3: May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc- binding lectin. Has affinity with GalNAc, GlcNAc, D-fucose, as mono/oligosaccharide and lipopolysaccharides from S.typhimurium and S.minnesota. Defects in FCN3 are the cause of ficolin 3 deficiency (FCN3D). FCN3D is a disorder characterized by immunodeficiency, recurrent infections, brain abscesses and recurrent warts on the fingers. Affected individuals have normal levels of lymphocytes, normal T-cell responses, and normal antibodies, but a selective deficient antibody response to pneumococcal polysaccharide vaccine. Belongs to the ficolin lectin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: collagen; extracellular region

Molecular Function: protein binding; carbohydrate binding; antigen binding

Biological Process: negative regulation of virion penetration into host cell; innate immune response; complement activation, lectin pathway; defense response to virus; recognition of apoptotic cell; complement activation

Disease: Immunodeficiency Due To Ficolin 3 Deficiency