Mouse anti-Human ZFP-42 / REX1 Polyclonal Antibody | anti-ZFP-42 / REX1 antibody

Anti-ZFP-42 / REX1 antibody

Cat. Num. AAA355187

• Gene Names: ZFP42; REX1; ZNF754
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry
• Purity: Protein A or Protein G purified
• Synonyms: ZFP-42 / REX1; Polyclonal Antibody; Anti-ZFP-42 / REX1 antibody; Monoclonal Anti- ZFP-42 / REX1; anti-ZFP-42 / REX1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG1
• Clone Number: Z4
• Purity/Purification: Protein A or Protein G purified
• Form/Format: Liquid

• Applicable Applications for anti-ZFP-42 / REX1 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: WB (1:1000)
• Immunogen: A synthetic peptide (conjugated with KLH) corresponding to the C-terminal of human ZFP-42.
• Storage Buffer: In TBS (pH7.4), 0.5% BSA, 25% Glycerol and 0.05% Sodium Azide
• Storage Buffer: In TBS (pH7.4), 0.5% BSA, 25% Glycerol and 0.05% Sodium Azide.
• Preparation and Storage: Store at 4 degree C after thawing (1 week). Aliquot and store at -20 degree C for long term (at least one year). Avoid repeated freeze and thaw cycles.

Western Blot (WB)

Related Product Information for anti-ZFP-42 / REX1 antibody

Zfp-42 (Rex1) is a known marker of pluripotency, and most notably found in undifferentiated embryonic stem cells. It is a 288-amino acid protein and has a calculated molecular mass of 32.3 kD. [1]It has been identified in the testicles, expressed at the spermatocytes in the more inner layers of the testicles. Rex1 has been found to be critically important in maintaining proliferative state in mesenchymal stem cells(MSC), while simultaneously preventing differentiation.

References

1. Hosler, B. A., LaRosa, G. J., Grippo, J. F., Gudas, L. J. Expression of REX-1, a gene containing zinc finger motifs, is rapidly reduced by retinoic acid in F9 teratocarcinoma cells. Molec. Cell. Biol. 9: 5623-5629, 1989.

NCBI and Uniprot Product Information

• NCBI GI #: 89179323
• NCBI GeneID: 132625
• NCBI Accession #: NP_777560.2
• NCBI GenBank Nucleotide #: NM_174900.3
• UniProt Accession #: Q96MM3; Q8WXE2; D3DP65
• Molecular Weight: 34,802 Da
• NCBI Official Full Name: zinc finger protein 42 homolog
• NCBI Official Synonym Full Names: ZFP42 zinc finger protein
• NCBI Official Symbol: ZFP42
• NCBI Official Synonym Symbols: REX1; ZNF754
• NCBI Protein Information: zinc finger protein 42 homolog; REX-1; hREX-1; zfp-42; zinc finger protein 754; REX1 transcription factor; reduced expression protein 1
• UniProt Protein Name: Zinc finger protein 42 homolog
• UniProt Gene Name: ZFP42
• UniProt Synonym Gene Names: REX1; ZNF754; Zfp-42; REX-1; hREX-1
• UniProt Entry Name: ZFP42_HUMAN

Uniprot Description

Function: Involved in the reprogramming of X-chromosome inactivation during the acquisition of pluripotency. Required for efficient elongation of TSIX, a non-coding RNA antisense to XIST. Binds DXPas34 enhancer within the TSIX promoter. Involved in ES cell self-renewal

By similarity.

Subcellular location: Nucleus

By similarity.

Tissue specificity: Expressed in kidney, epidermal keratinocytes, prostate epithelial cells, bronchial and small airway lung epithelial cells (at protein level). Expressed in malignant kidney and several carcinoma cell lines (at protein level). Expressed in embryonic stem cells, kidney, epidermal keratinocytes, prostate epithelial cells, bronchial and small airway lung epithelial cells. Expressed in embryonal carcinomas, seminomas, malignant kidney and several carcinoma cell lines. Ref.1 Ref.6 Ref.7 Ref.8

Induction: Down-regulated upon retinoic acid addition to F9 stem cells. Ref.7

Post-translational modification: Polyubiquitinated by RNF12, leading to proteasomal degradation

By similarity.

Sequence similarities: Belongs to the krueppel C2H2-type zinc-finger protein family.Contains 4 C2H2-type zinc fingers.