Human Inactive Dipeptidyl Peptidase 10 (DPP10) ELISA Kit | DPP10 elisa kit

Human Inactive Dipeptidyl Peptidase 10 (DPP10) ELISA Kit

Cat. Num. AAA9716068

• Gene Names: DPP10; DPL2; DPPY; DPRP3; DPRP-3
• Reactivity: Human
• Synonyms: Inactive Dipeptidyl Peptidase 10 (DPP10); Human Inactive Dipeptidyl Peptidase 10 (DPP10) ELISA Kit; DPP10; DPL2; DPPY; DPRP3; OTTHUMP00000203618; dipeptidyl peptidase 10; dipeptidyl peptidase IV-related protein 3; dipeptidyl peptidase X; dipeptidyl peptidase like protein 2; DPP10 elisa kit

• Reactivity: Human
• Specificity: Human Inactive dipeptidyl peptidase 10 (DPP10) ELISA Kit has high sensitivity and excellent specificity for detection of Human DPP10. No significant cross-reactivity or interference between Human DPP10 and analogues was observed.
• Sequence Length: 789

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Inactive dipeptidyl peptidase 10 (DPP10) Standard Curve.)

Related Product Information for DPP10 elisa kit

Description: This Human Inactive dipeptidyl peptidase 10 (DPP10) ELISA Kit employs a two-site sandwich ELISA to quantitate DPP10 in samples. An antibody specific for DPP10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DPP10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DPP10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DPP10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: DPP10 encodes a single-pass type II membrane protein that is a member of the S9B family in clan SC of the serine proteases. This protein has no detectable protease activity, most likely due to the absence of the conserved serine residue normally present in the catalytic domain of serine proteases. However, it does bind specific voltage-gated potassium channels and alters their expression and biophysical properties. Mutations in this gene have been associated with asthma. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. DPP10 lacks the active-site serine, which is substituted with a glycine residue. Database analysis suggested the presence of a second DPP10 transcript. DPP10 shares 48% and 51% amino acid identity with the short and long DPP6 isoforms, respectively.

NCBI and Uniprot Product Information

• NCBI GI #: 85787627
• NCBI GeneID: 57628
• NCBI Accession #: NP_001004360.2
• NCBI GenBank Nucleotide #: NP_001004360.2
• UniProt Accession #: Q8N608
• NCBI Official Full Name: inactive dipeptidyl peptidase 10 isoform short
• NCBI Official Synonym Full Names: dipeptidyl peptidase like 10
• NCBI Official Symbol: DPP10
• NCBI Official Synonym Symbols: DPL2; DPPY; DPRP3; DPRP-3
• NCBI Protein Information: inactive dipeptidyl peptidase 10
• UniProt Protein Name: Inactive dipeptidyl peptidase 10
• Protein Family: Inactive dipeptidyl peptidase
• UniProt Gene Name: DPP10
• UniProt Synonym Gene Names: DPRP3; KIAA1492
• UniProt Entry Name: DPP10_HUMAN

NCBI Description

This gene encodes a single-pass type II membrane protein that is a member of the S9B family in clan SC of the serine proteases. This protein has no detectable protease activity, most likely due to the absence of the conserved serine residue normally present in the catalytic domain of serine proteases. However, it does bind specific voltage-gated potassium channels and alters their expression and biophysical properties. Mutations in this gene have been associated with asthma. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]