Mouse anti-Human PSA Monoclonal Antibody | anti-PSA antibody

PSA

Cat. Num. AAA344077

• Reactivity: Human
• Synonyms: PSA; Monoclonal Antibody; anti-PSA antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Form/Format: Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
• Sequence Length: 235

• Application Notes: The recommended working dilution range is 1:100 - 1:200.
• Positive Control: Prostate, Prostate Carcinoma
• Principles and Procedures: Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. The process involves the addition of the stated antibody (primary antibody) to a tissue slide, followed by a secondary antibody (linked to an enzyme complex) which specifically binds to the primary antibody. A chromogenic substrate is then added which reacts with the enzyme complex, resulting in a colorimetric reaction at the site of the antigen. Results are interpreted using a light microscope.
• Reconstitution: The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.; The concentrated antibody requires dilution in the optimized buffer, to the recommended working dilution range.
• Specimen Collection and Preparation for Analysis: Each tissue section should be fixed with 10% neutral buffered formalin, cut to the applicable thickness (4um), and placed on a glass slide that is positively charged. The prepared slide may then be baked for a minimum of 30 minutes in a 53-65 degree C oven (do not exceed 24 hours).; Note: Performance evaluation has been shown on human tissues only. Variable results may occur due to extended fixation time or special processes of specific tissue preparations.
• Preparation and Storage: Store at 2-8 degree C. Do not freeze.; To ensure proper stability and delivery of the antibody after each run, replace the cap and immediately place the bottle in a refrigerator in an upright position.; Positive and negative controls should be simultaneously run with unknown specimens.

Immunohistochemistry (IHC)

(PSA [IHC654] on Prostate)

Related Product Information for anti-PSA antibody

Intended Use: The PSA [IHC654] antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalin-fixed, paraffin-embedded tissue sections using IHC test methods.
Description: Prostate-Specific Antigen (PSA) is a serine protease of the kallikrein family, that is produced by the prostate epithelium and epithelial lining of the periurethral glands. Although considered prostate-specific, PSA has also been detected in breast tissue, breast tumors, endometrium, adrenal neoplasms, and renal cell carcinomas. Anti-PSA can be used for differentiating high-grade prostate adenocarcinoma from high-grade urothelial carcinoma, as well as for determining the prostatic origin of carcinomas in non-prostate tissues. Anti-PSA recognizes primary and metastatic prostatic neoplasms, but not tumors of nonprostatic origin, and can be useful as an aid to confirm prostatic acinar cell origin in primary and metastatic carcinomas.

References

1. Polascik TJ, et al. J Urol. 1999; 162:293-306.
2. Stenman UH, et al. Semin Cancer Biol. 1999; 9:83-93.
3. Alanen KA, et al. Path Res Pract. 1996; 192:233-7.
4. Varma M, et al. Histopathology. 2005; 47:1-16.
5. Gallee MP, et al. Prostate. 1986; 9:33-45.
6. Hadji M, et al. Cancer. 1981; 48:1229-32.
7. Tazawa K, et. al. Path Intl. 1999; 49:500-5.
8. Siddiqui IA, et. al. Carcinogenesis. 2006; 27:833-9.
9. Ljung G, et. al. Prostate. 1997; 31:91-7.

NCBI and Uniprot Product Information

• NCBI GI #: 1178019134
• Molecular Weight: 26,870 Da
• NCBI Official Full Name: PSA
• UniProt Protein Name: PSA
• Protein Family: PsaJ-like protein asl3190
• UniProt Gene Name: PSA

Uniprot Description

CautionThe sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.