Western Blot (WB)
(Western Blot analysis of MTERFD1 on different lysates using anti-MTERFD1 antibody at 1/1,000 dilution. Positive control: Lane1: Hela Lane2: Mouse kidney tissue Lane3: K562)
Mouse anti-Human, Mouse MTERFD1 Monoclonal Antibody | anti-MTERFD1 antibody
MTERFD1 Monoclonal Antibody
Gene Names
MTERF3; CGI-12; MTERFD1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
ProA affinity purified
Synonyms
MTERFD1; Monoclonal Antibody; MTERFD1 Monoclonal Antibody; mTERF3; MTERF3; CGI-12; CGI 12; mitochondrial; Mitochondrial transcription termination factor 3; MTER1; MTERF domain containing 1; mTERF domain containing protein 1 mitochondrial; mTERF domain-containing protein 1; Mterfd1; Transcription termination factor 3; anti-MTERFD1 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1
Specificity
MTERFD1 monoclonal antibody detects endogenous levels of MTERFD1 protein.
Purity/Purification
ProA affinity purified
Form/Format
Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
417
Applicable Applications for anti-MTERFD1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50
IHC: 1:50-1:200
ICC: 1:50
Immunogen
Recombinant protein
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of MTERFD1 on different lysates using anti-MTERFD1 antibody at 1/1,000 dilution. Positive control: Lane1: Hela Lane2: Mouse kidney tissue Lane3: K562)
Western Blot (WB)
(Western Blot analysis of MTERFD1 on different lysates using anti-MTERFD1 antibody at 1/1,000 dilution. Positive control: Lane1: Hela Lane2: Mouse kidney tissue Lane3: K562)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MTERFD1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MTERFD1 antibody. Counter stained with hematoxylin.)
Related Product Information for anti-MTERFD1 antibody
MTERFD1 belongs to the MTERF family which consists of four members including MTERF1, MTERF2, MTERF3 and MTERF4. Previous research showed that MTERFD1 is a mitochondrial protein that interacts with the mitochondrial DNA promoter region and decreases transcription initiation in mammalian mitochondria. This negative regulation is likely important for fine-tuning mitochondria transcription in response to physiological demands. Human mitochondrial transcription termination factor domain 1 (MTERFD1) was mutated in many types of cancer. In vivo and in vitro data indicated that MTERFD1 gene possesses oncogenic properties.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48/38kDa
NCBI Official Full Name
transcription termination factor 3, mitochondrial isoform 1
NCBI Official Synonym Full Names
mitochondrial transcription termination factor 3
NCBI Official Symbol
MTERF3
NCBI Official Synonym Symbols
CGI-12; MTERFD1
NCBI Protein Information
transcription termination factor 3, mitochondrial
UniProt Protein Name
Transcription termination factor 3, mitochondrial
UniProt Gene Name
MTERF3
UniProt Synonym Gene Names
MTERFD1; mTERF3
UniProt Entry Name
MTEF3_HUMAN
Uniprot Description
MTERFD1: Binds promoter DNA and regulates initiation of transcription. Required for normal mitochondrial transcription, and for normal assembly of mitochondrial respiratory complexes. Required for normal mitochondrial function. Belongs to the mTERF family. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 8q22.1
Cellular Component: mitochondrion
Molecular Function: double-stranded DNA binding
Biological Process: transcription, DNA-dependent; negative regulation of transcription, DNA-dependent
Research Articles on MTERFD1
Similar Products
Product Notes
The MTERFD1 mterf3 (Catalog #AAA3014500) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MTERFD1 Monoclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MTERFD1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC). WB: 1:1,000-1:2,000 IHC: 1:50-1:200 ICC: 1:50. Researchers should empirically determine the suitability of the MTERFD1 mterf3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MTERFD1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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