Western Blot (WB)
(Western blot analysis of MTERFD1 in 3T3 cell lysate with MTERFD1 antibody at (A) 1 and (B) 2 μg/mL.)
Rabbit anti-Human, Mouse MTERFD1 Polyclonal Antibody | anti-MTERFD1 antibody
MTERFD1 Antibody
Gene Names
MTERF3; CGI-12; MTERFD1
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
MTERFD1 Antibody is affinity chromatography purified via peptide column.
Synonyms
MTERFD1; Polyclonal Antibody; MTERFD1 Antibody; CGI-12; mTERF3; mTERF domain-containing protein 1; mitochondrial; Mitochondrial transcription termination factor 3; MTERF domain containing 1; anti-MTERFD1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
At least three isoforms of MTERFD1 are known to exist. MTERFD1 antibody is predicted to not cross-react with other MTERFD protein family members.
Purity/Purification
MTERFD1 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
417
Applicable Applications for anti-MTERFD1 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
MTERFD1 Antibody can be used for detection of MTERFD1 by Western blot starting at 1 mug/mL. Antibody can also be used for Immunocytochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
MTERFD1 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human MTERFD1.
Buffer
MTERFD1 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
MTERFD1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
Western Blot (WB)
(Western blot analysis of MTERFD1 in 3T3 cell lysate with MTERFD1 antibody at (A) 1 and (B) 2 μg/mL.)
Western Blot (WB)
(Western blot analysis of MTERFD1 in 3T3 cell lysate with MTERFD1 antibody at (A) 1 and (B) 2 μg/mL.)
Immunocytochemistry (IHC)
(Immunocytochemistry of MTERFD1 in 3T3 cells with MTERFD1 antibody at 5 μg/mL.)
Immunocytochemistry (IHC)
(Immunocytochemistry of MTERFD1 in 3T3 cells with MTERFD1 antibody at 5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of MTERFD1 in 3T3 cells with MTERFD1 antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of MTERFD1 in 3T3 cells with MTERFD1 antibody at 20 μg/mL.)
Related Product Information for anti-MTERFD1 antibody
MTERFD1 Antibody: Members of the mTERF (mitochondrial transcription termination factor) family, are mitochondrial proteins that are believed to be transcription termination factors. MTERFD1 is composed of three leucine zippers that form a three-stranded coiled-coil that binds to DNA. It binds promoter DNA and acts as a mitochondrial transcription regulator. It has been suggested that only the phosphorylated form of MTERFD1 has transcription termination activity. A closely related mTERF family member, MTERFD3, is believed to be involved in cell cycle regulation and cell growth by modulating mitochondrial transcription.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 46 kDa
Observed: 42 kDa
Observed: 42 kDa
NCBI Official Full Name
transcription termination factor 3, mitochondrial isoform 1
NCBI Official Synonym Full Names
mitochondrial transcription termination factor 3
NCBI Official Symbol
MTERF3
NCBI Official Synonym Symbols
CGI-12; MTERFD1
NCBI Protein Information
transcription termination factor 3, mitochondrial; MTERF domain containing 1; mTERF domain-containing protein 1, mitochondrial
UniProt Protein Name
Transcription termination factor 3, mitochondrial
UniProt Gene Name
MTERF3
UniProt Synonym Gene Names
MTERFD1; mTERF3
UniProt Entry Name
MTEF3_HUMAN
Uniprot Description
MTERFD1: Binds promoter DNA and regulates initiation of transcription. Required for normal mitochondrial transcription, and for normal assembly of mitochondrial respiratory complexes. Required for normal mitochondrial function. Belongs to the mTERF family. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 8q22.1
Cellular Component: mitochondrion
Molecular Function: double-stranded DNA binding
Biological Process: transcription, DNA-dependent; negative regulation of transcription, DNA-dependent
Research Articles on MTERFD1
Similar Products
Product Notes
The MTERFD1 mterf3 (Catalog #AAA153562) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MTERFD1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MTERFD1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). MTERFD1 Antibody can be used for detection of MTERFD1 by Western blot starting at 1 mug/mL. Antibody can also be used for Immunocytochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the MTERFD1 mterf3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MTERFD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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